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Additional disinfection with a modified salt solution in a root canal model.

Suzette V van der Waal¹, Charlotte A M Oonk², Selma H Nieman²

¹Department of Preventive Dentistry, Academic Centre for Dentistry Amsterdam (ACTA), Gustav Mahlerlaan 3004, 1081 LA Amsterdam, The Netherlands; Department of Cariology, Endodontology and Pedodontology, Academic Centre for Dentistry Amsterdam (ACTA), Gustav Mahlerlaan 3004, 1081 LA Amsterdam, The Netherlands.

Journal of Dentistry

|August 4, 2015

Summary

A modified salt solution (MSS) effectively prevented bacterial regrowth in root canals, outperforming calcium hydroxide (Ca(OH)2) in preventing persistent Enterococcus faecalis infections after disinfection.

Keywords:

Calcium hydroxide Disinfection Endodontic Enterococcus faecalis Modified salt solution Root canal

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Area of Science:

Endodontics
Microbiology
Dental Materials Science

Background:

Persistent Enterococcus faecalis infections pose a challenge in endodontic treatment.
Sodium hypochlorite (NaOCl) is a standard irrigant, but additional disinfection methods are explored.
Calcium hydroxide (Ca(OH)2) is commonly used as an intracanal medicament.

Purpose of the Study:

To evaluate the disinfecting properties of a modified salt solution (MSS) and Ca(OH)2.
To compare their efficacy against Enterococcus faecalis in a non-direct-contact ex-vivo model.
To assess their ability to prevent bacterial regrowth after initial disinfection.

Main Methods:

Seventy-four infected root canals were treated with NaOCl irrigation, with or without subsequent MSS or Ca(OH)2 dressing.
Bacterial regrowth was assessed after incubation by sampling root canals.
Colony-forming units were counted, and regrowth was determined (no/yes).

Main Results:

MSS dressing significantly reduced bacterial regrowth compared to Ca(OH)2 after NaOCl irrigation (P=0.009).
After NaOCl irrigation alone, 14/19 roots showed regrowth.
MSS dressing resulted in regrowth in 6/20 roots, while Ca(OH)2 showed regrowth in 14/20 roots.

Conclusions:

The modified salt solution demonstrated effectiveness in preventing bacterial regrowth, indicating potential for eliminating persistent root canal bacteria.
Intracanal dressing with Ca(OH)2 did not offer additional disinfection benefits beyond NaOCl irrigation.
MSS shows promise as an adjunctive disinfection agent in endodontics.