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Updated: Apr 6, 2026

Use of Human Perivascular Stem Cells for Bone Regeneration

Use of Human Perivascular Stem Cells for Bone Regeneration

Published on: May 25, 2012

A computer-designed scaffold for bone regeneration within cranial defect using human dental pulp stem cells.

Doo Yeon Kwon¹, Jin Seon Kwon¹, Seung Hun Park¹

¹Department of Molecular Science and Technology, Ajou University, Suwon 443-759, Korea.

Scientific Reports

|August 4, 2015

Summary

This study demonstrates successful neo-bone regeneration in cranial defects using a computer-designed scaffold and human dental pulp stem cells. The biodegradable scaffold facilitated significant bone formation in vivo.

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Last Updated: Apr 6, 2026

Use of Human Perivascular Stem Cells for Bone Regeneration

Use of Human Perivascular Stem Cells for Bone Regeneration

Published on: May 25, 2012

Author Spotlight: Advancing Tissue Regeneration and Disease Modeling with Dental Pulp Stem Cells

Author Spotlight: Advancing Tissue Regeneration and Disease Modeling with Dental Pulp Stem Cells

Published on: May 5, 2023

Repair of a Critical-sized Calvarial Defect Model Using Adipose-derived Stromal Cells Harvested from Lipoaspirate

Repair of a Critical-sized Calvarial Defect Model Using Adipose-derived Stromal Cells Harvested from Lipoaspirate

Published on: October 31, 2012

Area of Science:

Biomaterials Science
Regenerative Medicine
Tissue Engineering

Background:

Computer-designed, solvent-free scaffolds offer advantages in customized manufacturing and in vivo safety.
Human dental pulp stem cells (hDPSCs) are abundant, proliferative, and can differentiate into osteoblasts.

Purpose of the Study:

To evaluate the efficacy of a computer-designed, solvent-free scaffold combined with hDPSCs for neo-bone regeneration in cranial defects.

Main Methods:

Fabrication of a computer-designed scaffold using solid freeform fabrication (SFF) with biodegradable polyesters (MPEG-(PLLA-co-PGA-co-PCL) or PLGC).
Implantation of the PLGC scaffold with hDPSCs and osteogenic factors (OF) into cranial bone defects in vivo.
Assessment of neo-bone formation using micro-computed tomography (micro-CT) and histology.

Main Results:

Significant neo-bone formation (>50%) was observed in cranial defects treated with the PLGC scaffold, hDPSCs, and OF.
The PLGC scaffold demonstrated gradual biodegradation, allowing for tracking of implanted material.
Micro-CT and histological analyses confirmed successful tissue-engineered bone regeneration.

Conclusions:

A computer-designed, solvent-free PLGC scaffold effectively supports neo-bone formation in cranial defects when combined with hDPSCs and OF.
This approach shows promise for bone tissue engineering applications.
The study confirms the potential of hDPSCs and custom scaffolds for regenerating bone defects.