Related Experiment Video
Updated: Apr 6, 2026
In vivo Structural Assessments of Ocular Disease in Rodent Models using Optical Coherence Tomography
Published on: July 24, 2020
Observation of white spot lesions using swept source optical coherence tomography (SS-OCT): in vitro and in vivo
Takahide Ibusuki1, Yuichi Kitasako, Alireza Sadr
1Cariology and Operative Dentistry, Department of Restorative Sciences, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University.
Abstract:
This study aimed to assess swept source optical coherence tomography (SS-OCT) for in vitro and in vivo detection of enamel white spot lesion (WSL). WSLs without surface breakdown on 33 extracted human posterior teeth were non-invasively scanned using SSOCT. The teeth were then cross-sectioned and imaged under confocal laser scanning microscope (CLSM) and light microscopy (LM). SS-OCT cross-sectional images were compared with CLSM and LM. WSL shapes in SS-OCT images closely corresponded to those of LM. There were significant correlations (p<0.001) in WSLs depth between SS-OCT and LM (r=0.92), SS-OCT and CLSM (r=0.80) and CLSM and LM (r=0.85). Six WSLs were also evaluated clinically using SS-OCT; clear in-depth images of these natural WSLs were obtained in vivo. SS-OCT appears to be an effective tool for observation of the internal structure of WSLs, enabling quantitative assessment of WSL depth. Such data can be considered in the clinical management of WSLs.
