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Related Experiment Video

Updated: Apr 6, 2026

Measuring Relative Insulin Secretion using a Co-Secreted Luciferase Surrogate
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Filter paper-based insulin-like growth factor assay.

Anne K Jensen1, Carrie Coleman2, David Stokes2

  • 1Division of Ophthalmology, The Children's Hospital of Philadelphia, Philadelphia, Pennsylvania; Scheie Eye Institute, Department of Ophthalmology, University of Pennsylvania Perelman School of Medicine, Philadelphia.

Journal of AAPOS : the Official Publication of the American Association for Pediatric Ophthalmology and Strabismus
|August 4, 2015
PubMed
Summary

This study validates a filter paper blood spot assay for insulin-like growth factor 1 (IGF-1), proving it reliable and feasible for retinopathy of prematurity (ROP) research. The method shows high accuracy and stability, supporting its use in clinical studies.

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Area of Science:

  • Biochemistry
  • Pediatric Ophthalmology
  • Clinical Diagnostics

Background:

  • Insulin-like growth factor 1 (IGF-1) plays a crucial role in development and is implicated in retinopathy of prematurity (ROP).
  • Accurate IGF-1 measurement is vital for ROP research, but traditional serum assays require venipuncture, posing challenges in neonates.
  • Filter paper blood spot assays offer a less invasive alternative for sample collection and transport.

Purpose of the Study:

  • To assess the validity, reliability, and feasibility of a filter paper blood spot assay for measuring IGF-1 in the context of ROP research.
  • To determine the stability of IGF-1 on filter paper under various storage conditions.
  • To evaluate the practical application of this assay in a cohort of premature infants undergoing ROP screening.

Main Methods:

  • Blood samples from 45 healthy children were divided into serum and filter paper spots.
  • IGF-1 levels were measured in both serum and blood spots using a commercial assay kit.
  • Filter paper samples were stored under different time and temperature conditions to assess stability.
  • Test-retest reliability was evaluated.
  • Feasibility was assessed through collection and analysis of 817 filter card samples from 74 premature infants in a second cohort study.

Main Results:

  • High intraclass correlation (0.97) was observed between blood spot and serum IGF-1 values, indicating excellent validity.
  • Low mean differences confirmed good test-retest reliability.
  • IGF-1 levels on filter paper remained stable for up to 25 days and across temperatures from 4°C to 37°C.
  • Successful collection, transport, and assay of 817 filter card samples from premature infants demonstrated feasibility.

Conclusions:

  • The filter paper blood spot assay for IGF-1 is a valid, reliable, and stable method for ROP research.
  • This minimally invasive technique is feasible for large-scale sample collection and analysis in premature infants.
  • The assay facilitates easier sample handling and transport, making it suitable for multicenter ROP studies and potentially for clinical diagnostics.