In vitro interaction of Stenotrophomonas maltophilia with human monocyte-derived dendritic cells
Emanuela Roscetto1, Laura Vitiello2, Rosa Muoio1
1Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Università Federico II , Napoli, Italy.
Abstract:
Stenotrophomonas maltophilia is increasingly identified as an opportunistic pathogen in immunocompromised, cancer and cystic fibrosis (CF) patients. Knowledge on innate immune responses to S. maltophilia and its potential modulation is poor. The present work investigated the ability of 12 clinical S. maltophilia strains (five from CF patients, seven from non-CF patients) and one environmental strain to survive inside human monocyte-derived dendritic cells (DCs). The effects of the bacteria on maturation of and cytokine secretion by DCs were also measured. S. maltophilia strains presented a high degree of heterogeneity in internalization and intracellular replication efficiencies as well as in the ability of S. maltophilia to interfere with normal DCs maturation. By contrast, all S. maltophilia strains were able to activate DCs, as measured by increase in the expression of surface maturation markers and proinflammatory cytokines secretion.
Insights
Stenotrophomonas maltophilia can survive within human dendritic cells, impacting their maturation. However, all strains effectively activate these immune cells, highlighting a complex interaction.
Area of Science:
- Immunology
- Microbiology
- Infectious Diseases
Background:
- Stenotrophomonas maltophilia is an opportunistic pathogen affecting immunocompromised, cancer, and cystic fibrosis (CF) patients.
- Understanding the innate immune response to S. maltophilia and its modulation is crucial.
Purpose of the Study:
- To investigate the survival of clinical and environmental S. maltophilia strains within human monocyte-derived dendritic cells (DCs).
- To assess the impact of S. maltophilia on DC maturation and cytokine secretion.
Main Methods:
- Human monocyte-derived dendritic cells (DCs) were exposed to 12 clinical and 1 environmental S. maltophilia strains.
- Bacterial survival, intracellular replication, DC maturation markers, and cytokine secretion were measured.
Main Results:
- S. maltophilia strains exhibited significant heterogeneity in their ability to be internalized and replicate within DCs.
- Variable interference with DC maturation was observed among different S. maltophilia strains.
- All tested S. maltophilia strains successfully activated DCs, evidenced by increased surface maturation markers and pro-inflammatory cytokine release.
Conclusions:
- S. maltophilia demonstrates diverse interactions with dendritic cells, including variable intracellular survival and modulation of maturation.
- Despite heterogeneous intracellular behavior, S. maltophilia consistently activates dendritic cells, indicating a conserved immune stimulatory effect.
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