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Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

18.7K
In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or...
18.7K
Enzyme-linked Receptors01:00

Enzyme-linked Receptors

89.1K
Enzyme-linked receptors are proteins that act as both receptor and enzyme, activating multiple intracellular signals. This is a large group of receptors that include the receptor tyrosine kinase (RTK) family. Many growth factors and hormones bind to and activate the RTKs.
Neurotrophin (NT) receptors are a family of RTKs, including trkA, trkB, and trkC (tropomyosin-related kinase) receptors. TrkA is specific for nerve growth factor (NGF), neurotrophin-6, and neurotrophin-7. TrkB binds...
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Related Experiment Video

Updated: Apr 6, 2026

Enzyme-linked Immunospot Assay ELISPOT: Quantification of Th-1 Cellular Immune Responses Against Microbial Antigens
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Enzyme-linked Immunospot Assay ELISPOT: Quantification of Th-1 Cellular Immune Responses Against Microbial Antigens

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Enzyme-Linked Immunosorbent Assays.

Peter V Hornbeck1

  • 1Cell Signaling Technology, Danvers, Massachusetts.

Current Protocols in Immunology
|August 4, 2015
PubMed
Summary
This summary is machine-generated.

This study details six Enzyme-Linked Immunosorbent Assay (ELISA) systems for detecting antibodies and antigens. These methods utilize enzyme-linked conjugates and substrate reactions to quantify analytes, offering versatile immunoassay solutions.

Keywords:
ELISAantibodyantigenenzyme-linked immunosorbant assaysolid-phase reactants

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Area of Science:

  • Immunology
  • Biochemistry
  • Assay Development

Background:

  • Enzyme-Linked Immunosorbent Assay (ELISA) is a widely used immunoassay technique.
  • Detection of specific antibodies, soluble antigens, and cell-surface antigens is crucial in diagnostics and research.
  • Standard ELISA protocols require optimization for specific applications.

Purpose of the Study:

  • To describe six distinct ELISA systems for diverse analyte detection.
  • To provide detailed protocols for preparing solid-phase reactants and enzyme conjugates.
  • To offer a method for optimizing ELISA performance and preparing alkaline phosphatase conjugates.

Main Methods:

  • Utilizing solid-phase reactants (adsorbed antigens/antibodies or cell-associated molecules) to capture analytes.
  • Employing enzyme-conjugated secondary or tertiary reactants for signal amplification.
  • Measuring product generated from chromogenic or fluorogenic substrate hydrolysis by bound enzyme conjugates.

Main Results:

  • Successful implementation of six different ELISA systems.
  • Quantification of analytes (antibodies, soluble antigens, cell-surface antigens) based on generated signal.
  • Demonstrated proportionality between product generated and analyte amount.

Conclusions:

  • The described ELISA systems provide versatile and sensitive methods for analyte detection.
  • Support protocols offer valuable tools for assay optimization and reagent preparation.
  • These ELISAs are adaptable for various research and diagnostic applications.