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Updated: Apr 6, 2026
Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy
Published on: March 5, 2017
Applications of Lipid Nanodiscs for the Study of Membrane Proteins by Surface Plasmon Resonance
Meg Trahey1,2, Mavis Jiarong Li3,2, Hyewon Kwon3
1Department of Biomedical and Pharmaceutical Sciences, University of Montana, Missoula, Montana.
Abstract:
Methods for the initial steps of surface plasmon resonance analysis of membrane proteins incorporated in lipid nanodiscs are described. Several types of Biacore sensor chips are available and require distinct strategies to immobilize proteonanodiscs on the chip surface. The procedures for immobilization on three of these chips (NTA, antibody coupled CM5, and L1) are described in this unit and results are demonstrated for a model system with cytochrome P4503A4 (CYP3A4) in nanodiscs binding to a polyclonal anti-CYP3A4 antibody. Advantages and disadvantages of each chip type are considered.
