Purification and characterization of a 94-kDa Pasteurella haemolytica antigen

A 94-kDa antigen of Pasteurella haemolytica Serotype 1, which was previously shown to elicit serum and nasal secretion antibody response to the bacterium, was purified and characterized. The antigen was purified by high performance liquid chromatography utilizing ion exchange, then size exclusion columns. It was a membrane protein that was copurified with 6-7% lipopolysaccharide. It had an isoelectric point of 4.6. Most other serotypes of P. haemolytica possessed a similar antigen.