Related Experiment Video
Updated: Apr 6, 2026
Enrichment and Detection of Clostridium perfringens Toxinotypes in Retail Food Samples
Published on: October 18, 2019
Comparative analysis of extractable proteins from Clostridium perfringens type A and type C strains showing varying
Pratistha Dwivedi1, Syed Imteyaz Alam1, Om Kumar2
1Biotechnology Division, Defence Research and Development Establishment, Gwalior 474002, India.
Abstract:
The prevailing scenario of bioterrorism warrants development of medical countermeasures with expanded coverage of select agents. Clostridium perfringens is a pathogen of medical, veterinary and military importance, and has been listed as Validated Biological Agent. We employed 2DE-MS approach to identify a total of 134 unique proteins (529 protein spot features) from the extractable proteome of four type A and type C strains. Proteins showing altered expression under host-simulated conditions from virulent type A strain (ATCC13124) were also elucidated. Significant among the differentially expressed proteins were elongation factor, molecular chaperones, ribosomal proteins, carbamoyl phosphate synthase, clpB protein, choloylglycine hydrolase, phosphopyruvate hydratase, and trigger factor. Predictive elucidation, of putative virulence associated proteins and sequence conservation pattern of selected candidates, was carried out using homologous proteins from other bacterial select agents to screen for the commonality of putative antigenic determinants. Pathogens (17 select agents) were observed to form three discrete clusters; composition of I and II being consistent in most of the phylogenetic reconstructions. This work provides a basis for further validation of putative candidate proteins as prophylactic agents and for their ability to provide protection against clusters of pathogenic select bacterial agents; aimed at mitigating the shadows of biothreat.
Insights
Developing new medical countermeasures against bioterrorism is crucial. This study identified key proteins in Clostridium perfringens, a dangerous pathogen, offering potential targets for broad-spectrum vaccines against select bacterial agents.
Area of Science:
- Microbiology
- Proteomics
- Bioterrorism Research
Background:
- Bioterrorism necessitates medical countermeasures against select agents like Clostridium perfringens.
- Clostridium perfringens is a significant pathogen with medical, veterinary, and military implications.
Purpose of the Study:
- To identify proteins from Clostridium perfringens strains using 2DE-MS.
- To investigate differentially expressed proteins under host-simulated conditions.
- To screen for conserved antigenic determinants among bacterial select agents.
Main Methods:
- Two-dimensional gel electrophoresis coupled with mass spectrometry (2DE-MS) was used to analyze the proteome.
- Proteins from four Clostridium perfringens strains (type A and C) were identified.
- Comparative analysis with homologous proteins from other bacterial select agents was performed.
Main Results:
- 134 unique proteins were identified from Clostridium perfringens.
- Key differentially expressed proteins included elongation factor, chaperones, and ribosomal proteins.
- Phylogenetic analysis revealed three distinct clusters among 17 select agents.
Conclusions:
- The identified proteins serve as potential candidates for prophylactic agents.
- This research provides a foundation for developing broad-spectrum vaccines against clusters of pathogenic select bacterial agents.
- The findings contribute to mitigating biothreats.
More Related Videos
09:26Identification of Antibacterial Immunity Proteins in Escherichia coli using MALDI-TOF-TOF-MS/MS and Top-Down Proteomic Analysis
Published on: May 23, 2021
08:04A New Approach for the Comparative Analysis of Multiprotein Complexes Based on 15N Metabolic Labeling and Quantitative Mass Spectrometry
Published on: March 13, 2014