Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

8.3K
Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
8.3K
DNA Isolation01:24

DNA Isolation

46.9K
DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
46.9K
Techniques for Isolation of Pure Cultures01:24

Techniques for Isolation of Pure Cultures

3.6K
Microorganisms are routinely cultured in the laboratory using various techniques to isolate, grow, and quantify them for further study. These methods rely on inoculating microorganisms into a suitable growth medium under aseptic conditions to prevent contamination. Depending on the objective, inoculation can involve direct transfer or the use of diluted bacterial suspensions as the inoculum.Streak-Plate Method for IsolationThe streak-plate method is a common technique for obtaining pure...
3.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Protocol for enhancing CRISPR-Cas9 genome editing using histone deacetylase inhibition and engineered virus-like particle delivery.

STAR protocols·2026
Same author

Lgmn targets two distinct GPCRs, PAR2 and µ-OR1, and induces cell death in acute lymphoblastic leukemia through an intracellular Ca²⁺ imbalance triggered by ER Ca²⁺ release.

Cell death discovery·2026
Same author

A recently evolved domain of the human ING1 epigenetic regulator targets mitochondria and induces senescence.

Cellular and molecular life sciences : CMLS·2026
Same author

Delineation and monitoring of the T cell repertoire of adoptive cell transfer product during the treatment of advanced melanoma.

Genome medicine·2025
Same author

FAME-CRISPR improves CRISPR-Cas9 genome editing via HDAC inhibition and engineered virus-like particle delivery.

Cell reports methods·2025
Same author

Functions of TIP60/NuA4 Complex Subunits in Cell Differentiation.

Cells·2025

Related Experiment Video

Updated: Apr 6, 2026

Nuclei Isolation from Fresh Frozen Brain Tumors for Single-Nucleus RNA-seq and ATAC-seq
06:22

Nuclei Isolation from Fresh Frozen Brain Tumors for Single-Nucleus RNA-seq and ATAC-seq

Published on: August 25, 2020

13.8K

Isolation of Nuclei.

Arash Nabbi1, Karl Riabowol2

  • 1Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Calgary, Calgary, Alberta T2N 4N1, Canada;

Cold Spring Harbor Protocols
|August 5, 2015
PubMed
Summary
This summary is machine-generated.

A new rapid method for isolating pure cell nuclei uses selective detergent dissolution and centrifugation. This technique minimizes contamination and protein modification, improving downstream molecular studies.

More Related Videos

Nuclei Isolation from Whole Tissue using a Detergent and Enzyme-Free Method
07:00

Nuclei Isolation from Whole Tissue using a Detergent and Enzyme-Free Method

Published on: June 24, 2020

27.3K
Author Spotlight: Nuclei Isolation from Mouse Cardiac Progenitor Cells for Epigenome and Gene Expression Profiling at Single-Cell Resolution
10:03

Author Spotlight: Nuclei Isolation from Mouse Cardiac Progenitor Cells for Epigenome and Gene Expression Profiling at Single-Cell Resolution

Published on: May 12, 2023

4.2K

Related Experiment Videos

Last Updated: Apr 6, 2026

Nuclei Isolation from Fresh Frozen Brain Tumors for Single-Nucleus RNA-seq and ATAC-seq
06:22

Nuclei Isolation from Fresh Frozen Brain Tumors for Single-Nucleus RNA-seq and ATAC-seq

Published on: August 25, 2020

13.8K
Nuclei Isolation from Whole Tissue using a Detergent and Enzyme-Free Method
07:00

Nuclei Isolation from Whole Tissue using a Detergent and Enzyme-Free Method

Published on: June 24, 2020

27.3K
Author Spotlight: Nuclei Isolation from Mouse Cardiac Progenitor Cells for Epigenome and Gene Expression Profiling at Single-Cell Resolution
10:03

Author Spotlight: Nuclei Isolation from Mouse Cardiac Progenitor Cells for Epigenome and Gene Expression Profiling at Single-Cell Resolution

Published on: May 12, 2023

4.2K

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Nuclei isolation is crucial for studying nuclear functions like protein localization and interactions.
  • Traditional methods are time-consuming and risk sample degradation or component loss.

Purpose of the Study:

  • To develop and present a rapid, high-purity nuclei isolation method.
  • To compare the new method with traditional protocols, highlighting advantages and limitations.

Main Methods:

  • Selective dissolution of the cytoplasmic membrane using a nonionic detergent.
  • Rapid centrifugation steps to separate nuclei from cellular debris.
  • Comparison with traditional glycerol/sucrose gradient centrifugation.

Main Results:

  • The new method provides rapid isolation of nuclei with high purity.
  • It minimizes cytoplasmic contamination, protein degradation, and post-isolation modifications.
  • Demonstrates comparable or superior results to traditional methods in reduced time.

Conclusions:

  • The developed detergent-based method offers an efficient and reliable approach for nuclei isolation.
  • This technique is suitable for various downstream applications requiring high-quality nuclear samples.
  • It addresses the limitations of conventional nuclei isolation protocols.