Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

CRISPR/Cas9 Genome Editing

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

CRISPR

CRISPR

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

ST3GAL3-mediated selective α2,3-sialylation of LDLR promotes vesicular stomatitis virus entry.

Biochimica et biophysica acta. General subjects·2026

Same author

Seal milk oligosaccharides rival human milk complexity and exhibit functional dynamics during lactation.

Nature communications·2025

Same author

Correction to "Understanding the Glycosylation Pathways Involved in the Biosynthesis of the Sulfated Glycan Ligands for Siglecs".

ACS chemical biology·2025

Same author

Uncommon N-Glycan Structures in Anhydrobiotic Tardigrades.

Molecular & cellular proteomics : MCP·2025

Same author

Structural insights into terminal arabinosylation of mycobacterial cell wall arabinan.

Nature communications·2025

Same author

Molecular basis of host recognition of human coronavirus 229E.

Nature communications·2025

Search research articles

Related Experiment Video

Updated: Apr 6, 2026

Sand Fly Phlebotomus papatasi Embryo Microinjection for CRISPR/Cas9 Mutagenesis

Sand Fly Phlebotomus papatasi Embryo Microinjection for CRISPR/Cas9 Mutagenesis

Published on: November 17, 2020

Modifying an Insect Cell N-Glycan Processing Pathway Using CRISPR-Cas Technology.

Hideaki Mabashi-Asazuma¹, Chu-Wei Kuo², Kay-Hooi Khoo²

¹Department of Molecular Biology, University of Wyoming , Laramie, Wyoming 82071, United States.

ACS Chemical Biology

|August 5, 2015

Summary

This summary is machine-generated.

Researchers used CRISPR-Cas9 to edit the fused lobes (FDL) gene in insect cells. This modification altered N-glycan processing, producing mammalian-type structures and confirming FDL

More Related Videos

Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera Hübner

Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera Hübner

Published on: July 1, 2021

Pupal and Adult Injections for RNAi and CRISPR Gene Editing in Nasonia vitripennis

Pupal and Adult Injections for RNAi and CRISPR Gene Editing in Nasonia vitripennis

Published on: December 4, 2020

Related Experiment Videos

Last Updated: Apr 6, 2026

Sand Fly Phlebotomus papatasi Embryo Microinjection for CRISPR/Cas9 Mutagenesis

Sand Fly Phlebotomus papatasi Embryo Microinjection for CRISPR/Cas9 Mutagenesis

Published on: November 17, 2020

Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera Hübner

Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera Hübner

Published on: July 1, 2021

Pupal and Adult Injections for RNAi and CRISPR Gene Editing in Nasonia vitripennis

Pupal and Adult Injections for RNAi and CRISPR Gene Editing in Nasonia vitripennis

Published on: December 4, 2020

Area of Science:

Glycobiology
Molecular Biology
Insect Cell Engineering

Background:

Insect and mammalian cells exhibit distinct N-glycan processing pathways.
The fused lobes (FDL) enzyme plays a crucial role in insect N-glycan trimming and elongation.
Understanding FDL function is key to explaining differences in glycoprotein glycosylation between insects and mammals.

Purpose of the Study:

To investigate the role of FDL in insect N-glycan processing using gene editing.
To assess the utility of CRISPR-Cas9 for modifying insect glycosylation pathways.
To generate a FDL-deficient insect cell line for future glycoengineering applications.

Main Methods:

Utilized the CRISPR-Cas9 gene editing system to target the fdl gene in Drosophila melanogaster S2 cells.
Analyzed N-glycan structures produced by wild-type and FDL-edited S2 cells.
Quantified nucleotide insertions/deletions and resulting glycan products.

Main Results:

CRISPR-Cas9 editing efficiently introduced site-specific mutations in the fdl gene.
FDL deficiency led to reduced production of insect-type paucimannosidic N-glycans (Man3GlcNAc2).
Mammalian-type complex N-glycans (GlcNAc2Man3GlcNAc2) were produced in FDL-deficient S2 cells.

Conclusions:

Confirms the critical role of FDL in distinguishing insect and mammalian N-glycan processing pathways.
Demonstrates the broad utility of CRISPR-Cas9 for analyzing and modifying insect glycosylation.
The generated FDL-deficient S2 cell line is a valuable tool for glycoengineering recombinant glycoproteins with defined structures.