Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

9.8K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
9.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Online scenario simulation teaching in airway management for undergraduate anesthesia students.

Frontiers in medicine·2025
Same author

DMF as an amine source: iron-catalyzed cyclization of 2<i>H</i>-azirines to imidazoles.

Chemical communications (Cambridge, England)·2024
Same author

[Response of Soil Nitrifier and Denitrifier Community and Activity to Elevated Atmospheric CO<sub>2</sub> Concentration and Temperature].

Huan jing ke xue= Huanjing kexue·2018
Same author

Novel rhodamine derivate as high selective detection lead sensor.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2014
Same author

A Selective Fluorescent Sensor for Hg(2+).

Journal of fluorescence·2013
Same author

[Screening of the binding peptides of Mycobacterium tuberculosis H(37)Rv].

Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases·2011

Related Experiment Video

Updated: Apr 6, 2026

Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy
14:04

Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy

Published on: April 25, 2021

6.3K

A novel rosamine based fluorescent sensor for Ag(+) recognition.

Lian-Qing Li1, Le-Jun Gao2

  • 1Department of Chemistry and Chemical Engineering, ShaanXi XueQian Normal University, Xi'an 710100, China.

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
|August 5, 2015
PubMed
Summary

A novel rosamine derivative (L) functions as a highly selective "turn-on" chemosensor for detecting silver ions (Ag+). This sensor demonstrates potential for sensitive silver recognition in solutions.

Keywords:
ChemosensorFluorescenceSilver ion

More Related Videos

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors
16:16

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors

Published on: September 13, 2013

15.9K
Qualitative Identification of Carboxylic Acids, Boronic Acids, and Amines Using Cruciform Fluorophores
09:46

Qualitative Identification of Carboxylic Acids, Boronic Acids, and Amines Using Cruciform Fluorophores

Published on: August 19, 2013

16.1K

Related Experiment Videos

Last Updated: Apr 6, 2026

Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy
14:04

Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy

Published on: April 25, 2021

6.3K
Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors
16:16

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors

Published on: September 13, 2013

15.9K
Qualitative Identification of Carboxylic Acids, Boronic Acids, and Amines Using Cruciform Fluorophores
09:46

Qualitative Identification of Carboxylic Acids, Boronic Acids, and Amines Using Cruciform Fluorophores

Published on: August 19, 2013

16.1K

Area of Science:

  • Organic Chemistry
  • Analytical Chemistry
  • Materials Science

Background:

  • Development of selective chemosensors is crucial for detecting specific metal ions.
  • Rosamine derivatives offer a promising scaffold for designing fluorescent probes.
  • Silver ion detection is important in environmental monitoring and biological systems.

Purpose of the Study:

  • To synthesize a novel rosamine derivative, L, as a turn-on chemosensor for silver ions.
  • To evaluate the selectivity and sensitivity of sensor L towards Ag+.
  • To investigate the binding interaction between sensor L and Ag+.

Main Methods:

  • Synthesis of the N-(9-(4-(bis(2-(ethylthio)ethyl)amino)phenyl)-6-(diethylamino)-3H-xanthen-3-ylidene)-N-ethylethanaminium hexafluorophosphate (L) compound.
  • Fluorescence spectroscopy to assess the 'turn-on' sensing behavior and selectivity.
  • 1H NMR titration experiments to study the binding mechanism with Ag+.

Main Results:

  • The synthesized sensor L demonstrated a highly selective 'turn-on' fluorescence response to Ag+.
  • Sensor L showed negligible response to other common metal cations (e.g., Cd2+, Cu2+, Hg2+, Na+, Mg2+, Ni2+, Pb2+, Fe3+, Zn2+).
  • A low detection limit in the 10(-7) M range was achieved for Ag+ detection.
  • 1H NMR titration confirmed the binding interaction between Ag+ and sensor L.

Conclusions:

  • The novel rosamine derivative L is an effective and highly selective chemosensor for silver ions.
  • The 'turn-on' fluorescence mechanism and low detection limit make L suitable for Ag+ sensing.
  • Sensor L holds potential as a material for practical silver ion recognition applications.