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Hematopoietic progenitors are required for proper development of coronary vasculature

Gentian Lluri1, Vincent Huang2, Marlin Touma3

  • 1Department of Molecular Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095, USA; Department of Medicine, Section of Cardiology, University of California, Los Angeles, Los Angeles, CA 90095, USA.

Journal of Molecular and Cellular Cardiology
|August 5, 2015
PubMed

Insights

Hematopoietic cells are crucial for coronary vessel development in the heart. Their absence leads to disorganized and underdeveloped coronary vasculature, suggesting a vital role in heart development.

Area of Science:

  • Developmental biology
  • Cardiovascular research
  • Hematopoiesis

Background:

  • Hematopoietic cells are present in the myocardium before coronary vessel formation.
  • The specific role of these early hematopoietic cells in coronary development remains unclear.

Purpose of the Study:

  • To investigate the necessity of pre-existing hematopoietic cells for coronary vasculature formation.
  • To elucidate the role of hematopoietic progenitors in heart development.

Main Methods:

  • Utilized Runx1 knockout and Vav1-cre; R26-DTA mouse models to create hematopoietic cell-deficient embryos.
  • Performed whole-mount and section stainings to analyze coronary vasculature.
  • Conducted ex vivo coronary explant experiments.

Main Results:

  • Runx1 knockout and Vav1-cre; R26-DTA embryos displayed disorganized and hypoplastic coronary microvasculature.
  • Ex vivo heart explants from these models showed impaired coronary formation.
  • Epicardial to mesenchymal transition was negatively affected in the absence of hematopoietic progenitors.

Conclusions:

  • Hematopoietic cells actively induce coronary growth, rather than being passively transported.
  • These findings reveal a novel mechanism regulating coronary vascular development.
Abstract

Keywords:
Cardiac developmentCoronary formationEMTEpicardiumHematopoietic progenitors

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