Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

23.0K
Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
23.0K
Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

839
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
839
Automated Microbial Diagnostics01:24

Automated Microbial Diagnostics

45
Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...
45

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Characterization of <i>Escherichia coli</i> isolates associated with bovine mastitis in Austria.

Frontiers in veterinary science·2026
Same author

Quantitative Multiplex Digital PCR with Fluorescence-Encoded Nanoreactor Beads.

Analytical chemistry·2026
Same author

[Community acquired pneumonia - an update].

MMW Fortschritte der Medizin·2026
Same author

Staphylococcus aureus sequence type 71 is a chimera that emerged twice.

BMC genomics·2026
Same author

Prevalence and genetic characterization of methicillin-resistant Staphylococcus aureus in Commercial aquaculture farms in Egypt.

Scientific reports·2026
Same author

The oral cavity as a reservoir for resistance- and hypervirulence-associated genes of <i>Klebsiella pneumoniae</i> in hospitalized patients.

Frontiers in microbiology·2026
Same journal

Multidrug-resistant Bacillus cereus in retail poultry meat: molecular insights into toxigenicity and antimicrobial resistance.

BMC microbiology·2026
Same journal

Morphology and multigene phylogeny reveal a new species of Kuehneromyces from Xizang, China.

BMC microbiology·2026
Same journal

Insights into antimicrobial resistance: dissemination and molecular epidemiology of carbapenemase and AmpC genes in uropathogenic Escherichia coli.

BMC microbiology·2026
Same journal

Comparison of library preparation protocols and bioinformatic pipelines in high-throughput 16S rRNA gene sequencing.

BMC microbiology·2026
Same journal

Solvent alternation of the antimicrobial, antioxidant, anticancer activities and chemical profiles of Trametes hirsuta (Wulfen) Lloyd.

BMC microbiology·2026
Same journal

Mortality outcomes and economic burden of antimicrobial-resistant bacterial bloodstream infections in a Nigerian tertiary hospital.

BMC microbiology·2026
See all related articles

Related Experiment Video

Updated: Apr 6, 2026

A Protein Microarray Assay for Serological Determination of Antigen-specific Antibody Responses Following Clostridium difficile Infection
09:12

A Protein Microarray Assay for Serological Determination of Antigen-specific Antibody Responses Following Clostridium difficile Infection

Published on: June 15, 2018

10.5K

DNA-Microarray-based Genotyping of Clostridium difficile.

Darius Gawlik1,2, Peter Slickers3,4, Ines Engelmann5,6

  • 1Institute for Medical Microbiology and Hygiene, Technische Universität Dresden, Dresden, Germany. darius.gawlik@web.de.

BMC Microbiology
|August 6, 2015
PubMed
Summary
This summary is machine-generated.

A new microarray accurately types Clostridium difficile strains, identifying toxin genes and resistance markers. This rapid method aids in controlling hospital outbreaks by quickly characterizing C. difficile isolates.

More Related Videos

Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray
07:35

Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray

Published on: April 25, 2014

13.3K
An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants
09:32

An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants

Published on: November 8, 2017

8.3K

Related Experiment Videos

Last Updated: Apr 6, 2026

A Protein Microarray Assay for Serological Determination of Antigen-specific Antibody Responses Following Clostridium difficile Infection
09:12

A Protein Microarray Assay for Serological Determination of Antigen-specific Antibody Responses Following Clostridium difficile Infection

Published on: June 15, 2018

10.5K
Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray
07:35

Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray

Published on: April 25, 2014

13.3K
An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants
09:32

An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants

Published on: November 8, 2017

8.3K

Area of Science:

  • Microbiology
  • Molecular Biology
  • Infectious Diseases

Background:

  • Clostridium difficile causes antibiotic-associated diarrhea and hospital outbreaks.
  • Molecular typing is crucial for managing C. difficile infections.
  • A microarray was developed to target key C. difficile genes.

Purpose of the Study:

  • To develop and validate a microarray for rapid C. difficile genotyping.
  • To characterize clinical isolates and detect toxin and resistance genes.
  • To correlate microarray profiles with established typing methods.

Main Methods:

  • Design of a microarray including toxin, antimicrobial resistance, and variable genes.
  • Linear amplification and biotin labeling of DNA from reference and clinical isolates.
  • Hybridization of amplicons to the microarray for gene and allele detection.

Main Results:

  • 37 distinct C. difficile profiles were identified from 234 clinical isolates and 6 reference strains.
  • Profiles primarily clustered according to MLST-defined clades.
  • Microarray results showed strong correlation with MLST-based clade assignments.

Conclusions:

  • The microarray assay enables rapid, high-throughput genotyping of C. difficile.
  • It facilitates accurate strain identification and toxin gene detection.
  • This method supports molecular epidemiology and outbreak surveillance of C. difficile.