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Induction of nasal polyps using house dust mite and Staphylococcal enterotoxin B in C57BL/6 mice
R Khalmuratova1, M Lee2, D W Kim3
1Department of Pharmacology, Seoul National University College of Medicine, Seoul, Republic of Korea.
Background:
The murine polyp model was developed previously using ovalbumin and Staphylococcus aureus enterotoxin B (SEB). Here, we established a model mimicking key aspects of chronic eosinophilic rhinosinusitis with nasal polyps using the house dust mite (HDM), a clinically relevant aeroallergen, co-administered with SEB. We assessed the inflammatory response and formation of nasal polypoid lesions in an experimental murine model using intranasal delivery of HDM and ovalbumin.
Methods:
After induction of HDM-induced allergic rhinosinusitis in C57BL/6 mice, SEB (10ng) was instilled into the nasal cavity of mice for eight weeks. Phosphate-buffered saline-challenged mice served as control. Histopathological changes were evaluated using haematoxylin and eosin for overall inflammation, Sirius red for eosinophils, and periodic acid-Schiff stain for goblet cells. The distribution of mast cells in mouse nasal tissue was determined by immunohistochemistry. Serum total IgE was measured using enzyme-linked immunosorbent assay.
Results:
Compared to mice treated with HDM only, the HDM+SEB-treated mice demonstrated nasal polypoid lesion formation and a significant increase in the number of secretory cells and eosinophilic infiltration. Moreover, mice challenged intranasally with HDM showed highly abundant mast cells in the nasal mucosa. In contrast, OVA+SEB-challenged mice showed a significantly lower degree of mast cell infiltration.
Conclusion:
We established an in vivo model of chronic allergic rhinosinusitis with nasal polypoid lesions using HDM aeroallergen. This study demonstrated that the HDM+SEB-induced murine polyp model could be utilised as a suitable model for nasal polyps, especially with both eosinophil and mast cell infiltration.
Insights
A new mouse model using house dust mite (HDM) and Staphylococcus aureus enterotoxin B (SEB) effectively mimics chronic rhinosinusitis with nasal polyps, showing significant eosinophil and mast cell infiltration.
Area of Science:
- Immunology
- Allergology
- Otorhinolaryngology
Background:
- Previous murine polyp models utilized ovalbumin and SEB.
- House dust mite (HDM) is a clinically relevant aeroallergen.
- A novel model using HDM and SEB was established to mimic chronic eosinophilic rhinosinusitis with nasal polyps.
Purpose of the Study:
- To establish and validate a murine model for chronic allergic rhinosinusitis with nasal polyps.
- To assess the inflammatory response and polypoid lesion formation induced by HDM and SEB.
Main Methods:
- C57BL/6 mice were challenged intranasally with HDM and SEB for eight weeks.
- Histopathology evaluated inflammation, eosinophils, and goblet cells.
- Mast cell distribution and serum IgE levels were quantified.
Main Results:
- HDM+SEB treatment induced nasal polypoid lesion formation and increased secretory cells.
- Significant eosinophilic infiltration and abundant mast cells were observed in HDM+SEB treated mice.
- Ovalbumin+SEB treatment resulted in lower mast cell infiltration compared to HDM+SEB.
Conclusions:
- A robust in vivo model for chronic allergic rhinosinusitis with nasal polyps was established using HDM aeroallergen.
- The HDM+SEB murine model is suitable for studying nasal polyps, particularly those involving eosinophil and mast cell infiltration.
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