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A procedure for eliminating interferences in the lowry method of protein determination
F Rodríguez-Vico1, M Martínez-Cayuela, E García-Peregrín
1Department of Biochemistry and Molecular Biology, University of Granada, Spain.
Abstract:
A modification of the Lowry assay for the quantitative protein measurement in the presence of interfering materials has been developed. The method is based on a precipitation with a single-phase hexane:isopropanol solvent system and later resuspension of protein pellets with sodium dodecyl sulfate and deoxycholate. The new procedure eliminates the interference caused by Triton X-100, phospholipids, or dithiothreitol providing yields higher than 95% and seems to be especially suitable for protein determination on membrane preparations in samples with small volumes and/or very low protein concentrations.