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Related Experiment Videos

Development of menaquinone-7 enriched nutraceutical: inside into medium engineering and process modeling.

Rishipal Singh1, Alka Puri1, Bibhu Prasad Panda1

  • 1Microbial and Pharmaceutical Biotechnology Laboratory, Centre for Advanced Research in Pharmaceutical Science, Faculty of Pharmacy, Jamia Hamdard, New Delhi, 110062 India.

Journal of Food Science and Technology
|August 6, 2015
PubMed
Summary

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Clinically Relevant Drug Product Specifications: Methods of Establishment01:29

Clinically Relevant Drug Product Specifications: Methods of Establishment

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Product specifications define the acceptable quality of a pharmaceutical product by ensuring identity, purity, potency, and strength. These specifications serve as benchmarks during development, manufacturing, and post-approval quality control. Clinically relevant specifications are particularly important because they directly relate to a drug's safety and efficacy in clinical use.Dissolution studies are critical biopharmaceutic tools that link in vitro behavior to in vivo performance. They...
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This study optimized solid-state fermentation of Bacillus subtilis to enhance menaquinone 7 (MK-7) production. Modified soybean medium significantly increased MK-7 yield within 24 hours, achieving high concentrations.

Area of Science:

  • Biotechnology
  • Microbial Fermentation
  • Nutritional Biochemistry

Background:

  • Menaquinone 7 (MK-7) is a vital nutrient produced via fermentation, primarily by Bacillus subtilis species.
  • Optimizing fermentation conditions is crucial for efficient and large-scale MK-7 production.

Purpose of the Study:

  • To enhance menaquinone 7 (MK-7) production using Bacillus subtilis NCIM 2708.
  • To identify optimal nutritional components for increased MK-7 yield under solid-state fermentation.
  • To achieve high MK-7 concentration in a reduced fermentation time.

Main Methods:

  • Solid-state fermentation using Bacillus subtilis NCIM 2708 with a modified soybean medium.
  • Investigation of nine nutritional components including glycerol, mannitol, dextrose, sucrose, yeast extract, malt extract, K2HPO4, MgSO4.7H2O, and CaCl2.
Keywords:
Medium engineeringMenaquinone-7Solid state fermentationSoybean

Related Experiment Videos

  • Utilizing Design Expert 7.1 software for prediction modeling.

    • Main Results:

    • The highest observed MK-7 concentration was 39.039 μg/g after 24 hours of fermentation.
    • Optimized medium components included soybean (20 g), glycerol (40 ml/kg), mannitol (60 g/kg), yeast extract (4 g/kg), malt extract (8 g/kg), and calcium chloride (4 g/kg).
    • A maximum MK-7 production of 56.757 μg/g was predicted by Design Expert software, showing 68.78% model validity.

    Conclusions:

    • The study successfully optimized a soybean-based medium for enhanced MK-7 production via solid-state fermentation.
    • The findings demonstrate the potential for rapid and high-yield MK-7 synthesis using Bacillus subtilis.
    • The predictive model offers a reliable tool for further optimization and scale-up of MK-7 fermentation.