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Protective Polymer Coatings for High-Throughput, High-Purity Cellular Isolation.

Gabriela Romero, Jacob J Lilly, Nathan S Abraham1

  • 1§Department of Chemical Engineering, University of Massachusetts Amherst, Amherst, Massachusetts 01003, United States.

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|August 6, 2015
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Summary

This study introduces polymer coatings for high-speed cell sorting, enabling ultrahigh purity cell populations for cell-based therapies. This breakthrough overcomes limitations in current sorting methods, paving the way for treating widespread diseases.

Keywords:
cell isolationcoatingsphotopolymerizationpolymerprotein expressionsorting

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Area of Science:

  • Biotechnology
  • Materials Science
  • Cell Biology

Background:

  • Cell-based therapies hold promise for treating diseases.
  • Current cell sorting methods lack high throughput and purity.
  • Limitations hinder the application of cell therapies for widespread diseases.

Purpose of the Study:

  • To develop a high-throughput method for ultrahigh purity cell sorting.
  • To utilize protective polymer coatings for cell enrichment.
  • To enable advanced cell-based therapies for prevalent conditions.

Main Methods:

  • Coating antigen-positive cells with biocompatible hydrogels.
  • Utilizing surfactant solutions to lyse unprotected cells.
  • Employing orthogonal photochemistry for polymer coating removal.

Main Results:

  • Achieved >50% yield of viable, functional cells post-sorting.
  • Enriched minority cell populations from blood to >99% purity.
  • Completed the entire batch process in under 1 hour with minimal equipment cost (<$2000).

Conclusions:

  • Protective polymer coatings offer a novel, high-speed cell enrichment strategy.
  • The method yields viable, functional cells suitable for cell-based therapies.
  • The process is scalable and cost-effective, addressing limitations in current cell sorting technologies.