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Following the Dynamics of Structural Variants in Experimentally Evolved Populations

Following the Dynamics of Structural Variants in Experimentally Evolved Populations

Published on: February 3, 2023

The Evolutionary Potential of Phenotypic Mutations.

Hayato Yanagida¹, Ariel Gispan², Noam Kadouri²

¹Department of Biological Chemistry, Weizmann Institute of Science, Rehovot, Israel.

|August 6, 2015

Summary

This summary is machine-generated.

Translational errors create protein diversity, driving evolutionary adaptation. These errors, like frameshifts, can be genetically imprinted, enhancing future evolutionary potential.

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Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli

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Published on: March 16, 2011

Area of Science:

Molecular Biology
Evolutionary Biology
Yeast Genetics

Background:

Phenotypic mutations (errors in protein synthesis) are far more common than genetic mutations.
Phenotypic variability can influence evolutionary trajectories.
The evolution of Saccharomyces cerevisiae IDP3 involved gene duplication and peroxisomal targeting.

Purpose of the Study:

To provide direct evidence that translational errors contribute to genetic and evolutionary adaptations.
To investigate the evolutionary origins of Saccharomyces cerevisiae IDP3 and its peroxisomal localization.
To explore the role of translational frameshifts and cryptic signals in evolution.

Main Methods:

Analysis of Saccharomyces cerevisiae IDP3 evolution post-whole genome duplication.
Investigation of +1 translational frameshifts bypassing stop codons to reveal cryptic 3'-UTR signals.
Bioinformatic screening of yeast genomes for putative cryptic signals.
Laboratory evolution experiments to study the imprinting of frameshifts via genetic deletions.

Main Results:

Pre-duplicated cytosolic IDPs show partial peroxisomal localization due to +1 frameshifts uncovering cryptic signals.
Other NADPH-producing enzymes, like PYC1, may also localize to peroxisomes via similar cryptic signals.
Laboratory evolution rapidly imprints translational frameshifts through genetic single-base deletions.
Sequences promoting frameshifts are also prone to genetic deletions, linking phenotypic variability to genetic adaptation.

Conclusions:

Translational errors and resulting phenotypic variability can precede and facilitate genetic adaptation.
Cryptic 3'-UTR sequences revealed by frameshifts provide immediate adaptive benefits and enhance future evolutionary potential.
This mechanism highlights a novel pathway linking protein synthesis fidelity to genome evolution.