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Related Experiment Videos

Dissimilatory nitrate reduction by Propionibacterium acnes.

C Allison1, G T Macfarlane

  • 1Medical Research Council, Dunn Clinical Nutrition Centre, Cambridge, United Kingdom.

Applied and Environmental Microbiology
|November 1, 1989
PubMed
Summary

Propionibacterium acnes P13 utilizes nitrate for growth, producing nitrite or nitrous oxide depending on pH. This process, particularly nitrate to nitrite reduction, is energetically favorable and may serve as a detoxification mechanism.

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Area of Science:

  • Microbiology
  • Environmental Science

Background:

  • Propionibacterium acnes is found in human feces.
  • Understanding its metabolic capabilities is crucial for human health and environmental studies.

Purpose of the Study:

  • To investigate the dissimilatory nitrate reduction pathways in Propionibacterium acnes P13.
  • To determine the influence of environmental factors like pH and electron donors on these pathways.
  • To assess the energetic favorability and potential toxicological role of nitrate reduction.

Main Methods:

  • Isolation and cultivation of Propionibacterium acnes P13.
  • Enzyme assays for nitrate and nitrite reductases.
  • Batch and chemostat cultures under varying pH conditions.
  • Analysis of growth rates, yields, and fermentation products.

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Main Results:

  • Propionibacterium acnes P13 possesses particulate nitrate reductase and soluble nitrite reductase, utilizing reduced viologen dyes as electron donors.
  • Nitrate enhanced growth rates and yields, with reduction products (nitrite or nitrous oxide) being pH-dependent.
  • Nitrite at low concentrations initially inhibited growth but was detoxified via reduction to nitrous oxide.
  • Dissimilatory nitrate reduction to nitrite was energetically favorable, increasing acetate to propionate ratios.

Conclusions:

  • Propionibacterium acnes P13 performs dissimilatory nitrate reduction, with products influenced by pH.
  • Nitrate reduction is an energetically beneficial process for this bacterium.
  • Denitrification in P. acnes may function as a detoxification pathway for nitrite.