Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Infrared (IR) Spectroscopy: Overview01:09

Infrared (IR) Spectroscopy: Overview

7.4K
When electromagnetic radiation passes through a material, atoms or molecules transition from a lower to a higher energy state by absorbing radiation corresponding to the energy difference between the two states. The absorption of infrared (IR) radiation causes transitions between vibrational energy levels in a molecule. Therefore, IR spectroscopy is a useful analytical tool for determining the molecular structure of molecules.
Different compounds display unique properties due to their...
7.4K
IR Frequency Region: Fingerprint Region01:03

IR Frequency Region: Fingerprint Region

2.3K
IR spectra are divided into two main regions: the diagnostic region and the fingerprint region. The diagnostic region of the spectrum lies above 1500 cm−1. The absorptions resulting from single-bond vibrations of the N–H, C–H, and O–H stretch at higher wavenumbers and appear on the left side of the spectrum. The stretching absorptions of the C≡C and C≡N occur between 2100–2300 cm−1. In contrast, those arising from stretching absorptions of the...
2.3K
IR and UV–Vis Spectroscopy of Aldehydes and Ketones01:29

IR and UV–Vis Spectroscopy of Aldehydes and Ketones

7.9K
Infrared spectroscopy, also known as vibrational spectroscopy, is mainly used to determine the types of bonds and functional groups in molecules. In aldehydes and ketones, the carbonyl (C=O) bond shows an absorption around 1710 cm-1. The C=O bond vibration of an aldehyde occurs at lower frequencies than that of a ketone. In addition to the C=O absorption in an aldehyde, the aldehydic C–H bond also gives two peaks in the 2700–2800 cm-1 range. This absorption, coupled with the...
7.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Role of the Pediatrician to Promote Effective Approaches for Child and Adolescent Nutrition in Schools: Policy Statement.

Pediatrics·2026
Same author

Perspective: Improving Neonatal Registered Dietitian Nutritionist Staffing, Utilization, and Compensation.

Advances in nutrition (Bethesda, Md.)·2025
Same author

Role of the neonatal registered dietitian nutritionist in Canada: A description of staffing and a comparison to practices in the United States.

Nutrition in clinical practice : official publication of the American Society for Parenteral and Enteral Nutrition·2024
Same author

US neonatal intensive care unit registered dietitian nutritionists salary description and correlates: results of a survey.

Journal of human nutrition and dietetics : the official journal of the British Dietetic Association·2024
Same author

Characteristics of fecal calprotectin as an early marker for suspected necrotizing enterocolitis in newborns exclusively fed maternal breast milk: a case-control study.

Proceedings (Baylor University. Medical Center)·2024
Same author

The Role of the Neonatal Registered Dietitian Nutritionist: Past, Present, and Future.

Clinics in perinatology·2023
Same journal

Assessment of sarcopenia in patients with Takayasu arteritis: A prospective cross-sectional observational study.

Nutrition in clinical practice : official publication of the American Society for Parenteral and Enteral Nutrition·2026
Same journal

Timing It Right theory-based staged video education for patients on home enteral nutrition: A randomized controlled trial.

Nutrition in clinical practice : official publication of the American Society for Parenteral and Enteral Nutrition·2026
Same journal

Feeding practices and nutritional management of infants with transient and congenital hyperinsulinism: A tertiary single-center retrospective study.

Nutrition in clinical practice : official publication of the American Society for Parenteral and Enteral Nutrition·2026
Same journal

Late-onset copper and zinc deficiency following Roux-en-Y gastric bypass (RYGB): A case report and review of the literature.

Nutrition in clinical practice : official publication of the American Society for Parenteral and Enteral Nutrition·2026
Same journal

Impact of body mass index on length of stay in patients with inflammatory bowel disease: A retrospective study.

Nutrition in clinical practice : official publication of the American Society for Parenteral and Enteral Nutrition·2026
Same journal

Malnutrition and clinical correlates in dementia with Lewy bodies: A retrospective cross-sectional study.

Nutrition in clinical practice : official publication of the American Society for Parenteral and Enteral Nutrition·2026
See all related articles

Related Experiment Video

Updated: Apr 5, 2026

High-definition Fourier Transform Infrared FT-IR Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology
11:05

High-definition Fourier Transform Infrared FT-IR Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology

Published on: January 21, 2015

34.1K

Human Milk Analysis Using Mid-Infrared Spectroscopy.

Sharon Groh-Wargo1, Jennifer Valentic2, Sharmeel Khaira2

  • 1Department of Pediatrics, Case Western Reserve University at MetroHealth Medical Center, Cleveland, Ohio Department of Nutrition, Case Western Reserve University at MetroHealth Medical Center, Cleveland, Ohio sgrohwargo@metrohealth.org.

Nutrition in Clinical Practice : Official Publication of the American Society for Parenteral and Enteral Nutrition
|August 7, 2015
PubMed
Summary
This summary is machine-generated.

Mid-infrared (MIR) spectroscopy accurately assesses macronutrient content in preterm infant human milk, aiding individualized nutrition plans. Further research is needed due to small sample size.

Keywords:
enteral nutritionhuman milkneonatesnewborn infant

More Related Videos

Milk Collection in the Rat Using Capillary Tubes and Estimation of Milk Fat Content by Creamatocrit
07:38

Milk Collection in the Rat Using Capillary Tubes and Estimation of Milk Fat Content by Creamatocrit

Published on: December 16, 2015

13.3K
Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization IR-MALDESI
10:47

Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization IR-MALDESI

Published on: March 24, 2016

10.0K

Related Experiment Videos

Last Updated: Apr 5, 2026

High-definition Fourier Transform Infrared FT-IR Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology
11:05

High-definition Fourier Transform Infrared FT-IR Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology

Published on: January 21, 2015

34.1K
Milk Collection in the Rat Using Capillary Tubes and Estimation of Milk Fat Content by Creamatocrit
07:38

Milk Collection in the Rat Using Capillary Tubes and Estimation of Milk Fat Content by Creamatocrit

Published on: December 16, 2015

13.3K
Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization IR-MALDESI
10:47

Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization IR-MALDESI

Published on: March 24, 2016

10.0K

Area of Science:

  • Neonatal Nutrition
  • Analytical Chemistry
  • Biomedical Engineering

Background:

  • Human milk composition varies, necessitating fortification for preterm infants.
  • Accurate milk analysis is crucial for tailoring nutrition for high-risk newborns.
  • Mid-infrared (MIR) spectroscopy offers a rapidly evolving technology for milk analysis.

Purpose of the Study:

  • To compare the accuracy of MIR spectroscopy with traditional laboratory methods for human milk macronutrient analysis.
  • To evaluate MIR spectroscopy as a tool for assessing the nutritional content of milk from mothers of low-birth-weight infants.

Main Methods:

  • Human milk samples from mothers of infants <2 kg birth weight were collected.
  • Macronutrients (protein, fat, lactose) were analyzed using MIR spectroscopy and reference laboratory methods (Kjeldahl, Mojonnier, HPLC).
  • Statistical comparisons included intraclass correlation coefficients, Bland-Altman plots, and paired t tests.

Main Results:

  • No significant differences were found in macronutrient content between MIR and reference laboratory analyses.
  • MIR spectroscopy demonstrated comparable results to established laboratory techniques for protein, fat, and lactose.

Conclusions:

  • MIR spectroscopy provides a reliable method for determining macronutrient content in expressed human milk from mothers of preterm infants.
  • While promising, the study's small sample size warrants caution. Lactose measurement requires further refinement due to oligosaccharide interference.
  • Human milk analysis via MIR holds potential for developing individualized fortification strategies for vulnerable infants.