Related Experiment Videos
[Isolation and purification of biopolymers using an affinity chromatography method. X. A biospecific approach to
Bioorganicheskaia Khimiia
|November 1, 1989
Abstract:
A biospecific method for one-stage isolation of collagen peptides containing the fibronectin binding site is proposed. alpha 1CB7-peptide of the type I collagen cyanogen bromide cleavage was isolated by means of affinity chromatography on adsorbents containing an immobilized gelatin-binding domain (45 kDa) of fibronectin. The method allows one to obtain, in a short time, highly purified preparation of alpha 1CB7-peptide necessary for biochemical studies.