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Published on: December 8, 2023
Quantification of maltol in Korean ginseng (Panax ginseng) products by high-performance liquid chromatography-diode
Hyun Cheol Jeong1, Hee-Do Hong1, Young-Chan Kim1
1Ginseng Research Center, Korea Food Research Institute, Seongnam, Gyeonggi 463-746, Korea.
Background:
Maltol, as a type of phenolic compounds, is produced by the browning reaction during the high-temperature treatment of ginseng. Thus, maltol can be used as a marker for the quality control of various ginseng products manufactured by high-temperature treatment including red ginseng. For the quantification of maltol in Korean ginseng products, an effective high-performance liquid chromatography-diode array detector (HPLC-DAD) method was developed.
Materials And Methods:
The HPLC-DAD method for maltol quantification coupled with a liquid-liquid extraction (LLE) method was developed and validated in terms of linearity, precision, and accuracy. An HPLC separation was performed on a C18 column.
Results:
The LLE methods and HPLC running conditions for maltol quantification were optimized. The calibration curve of the maltol exhibited good linearity (R (2) = 1.00). The limit of detection value of maltol was 0.26 μg/mL, and the limit of quantification value was 0.79 μg/mL. The relative standard deviations (RSDs) of the data of the intra- and inter-day experiments were <1.27% and 0.61%, respectively. The results of the recovery test were 101.35-101.75% with an RSD value of 0.21-1.65%. The developed method was applied successfully to quantify the maltol in three ginseng products manufactured by different methods.
Conclusion:
The results of validation demonstrated that the proposed HPLC-DAD method was useful for the quantification of maltol in various ginseng products.
