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Related Experiment Video

Updated: Apr 5, 2026

Clinical Examination Protocol to Detect Atypical and Classical Scrapie in Sheep

Clinical Examination Protocol to Detect Atypical and Classical Scrapie in Sheep

Published on: January 19, 2014

Transgenic Rabbits Expressing Ovine PrP Are Susceptible to Scrapie.

Pierre Sarradin¹, Céline Viglietta², Claude Limouzin³

¹INRA-Université de Tours, UMR1282, Infectiologie et Santé Publique, ISP, Nouzilly, France; INRA, UE1277, Plate-Forme d'Infectiologie Expérimentale, PFIE, Nouzilly, France.

|August 7, 2015

Summary

Transmissible spongiform encephalopathies (TSEs) are neurodegenerative diseases caused by prions. Transgenic rabbits expressing ovine prion protein developed TSEs, indicating rabbit resistance is not due to non-prion genetic factors.

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Last Updated: Apr 5, 2026

Clinical Examination Protocol to Detect Atypical and Classical Scrapie in Sheep

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Published on: January 19, 2014

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Procedures for Identifying Infectious Prions After Passage Through the Digestive System of an Avian Species

Procedures for Identifying Infectious Prions After Passage Through the Digestive System of an Avian Species

Published on: November 6, 2013

Area of Science:

Neuroscience
Molecular Biology
Veterinary Medicine

Background:

Transmissible spongiform encephalopathies (TSEs) are fatal neurodegenerative diseases.
Prions, misfolded prion proteins (PrPSc), cause TSEs by interacting with normal cellular prion proteins (PrPC).
Rabbit species exhibit significant resistance to prion diseases, hindering research.

Purpose of the Study:

To investigate the molecular basis of rabbit resistance to prion diseases.
To determine if altering prion protein genotype can overcome this resistance.

Main Methods:

Generated transgenic rabbits expressing the susceptible ovine prion protein (PrP) allele (V136R154Q171).
Inoculated transgenic and wild-type rabbits intracerebrally with scrapie prions.
Monitored animals for TSE development and analyzed brain tissue for prion presence.

Main Results:

All transgenic rabbits developed TSEs within 6-8 months post-inoculation.
Wild-type rabbits remained healthy for over 700 days.
Only ovine PrPSc, not rabbit PrPC, was detected in the brains of diseased transgenic animals.

Conclusions:

Rabbit resistance to prion infection is not determined by non-prion genetic factors.
The prion protein gene (PRNP) genotype plays a crucial role in determining susceptibility to TSEs.
Transgenic models expressing susceptible PrP alleles can overcome species barriers for prion diseases.