Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

1.0K
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
1.0K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

14.8K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
14.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dual-Function Metal-Phenolic Network-Capped Starch Nanoparticles for Postharvest Pesticide Removal and Produce Preservation.

ACS nano·2026
Same author

High-fidelity single-pixel imaging through scattering media using quantum-state encoded illumination.

Optics letters·2025
Same author

Single-Cell Identification and Characterization of Viable but Nonculturable <i>Campylobacter jejuni</i> Using Raman Optical Tweezers and Machine Learning.

Analytical chemistry·2025
Same author

Phosphorylation of RyR2 simultaneously expands the dyad and rearranges the tetramers.

The Journal of general physiology·2024
Same author

hiPSC-derived cardiomyocytes as a model to study the role of small-conductance Ca<sup>2+</sup>-activated K<sup>+</sup> (SK) ion channel variants associated with atrial fibrillation.

Frontiers in cell and developmental biology·2024
Same author

3D structured illumination microscope using a spinning disk [Invited].

Biomedical optics express·2023

Related Experiment Video

Updated: Apr 5, 2026

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules
10:20

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Published on: September 5, 2019

8.9K

Single molecule localization deep within thick cells; a novel super-resolution microscope.

Reza Tafteh1, David R L Scriven2, Edwin D W Moore2

  • 1Department of Chemistry, University of British Columbia, Vancouver, British Columbia, V6T 1Z1, Canada.

Journal of Biophotonics
|August 8, 2015
PubMed
Summary
This summary is machine-generated.

A new 3D imaging system provides drift-free microscopy deep within cells. Depth-dependent calibration reveals the true spread of cellular structures like ryanodine receptors, improving accuracy.

Keywords:
cardiomyocytedrift correctionhigh-density localizationryanodine receptorsingle-molecule localization

More Related Videos

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment
07:12

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment

Published on: January 6, 2026

673
Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells
11:06

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

9.2K

Related Experiment Videos

Last Updated: Apr 5, 2026

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules
10:20

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Published on: September 5, 2019

8.9K
Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment
07:12

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment

Published on: January 6, 2026

673
Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells
11:06

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

9.2K

Area of Science:

  • Biophysics
  • Cell Biology
  • Microscopy

Background:

  • Deep-cell imaging is challenging due to Z-axis distortions in conventional microscopy.
  • Accurate 3D reconstruction requires accounting for depth-dependent optical aberrations.

Purpose of the Study:

  • To develop a novel 3D single-molecule localization microscopy (SMLM) system for high-accuracy imaging deep within cells.
  • To address Z-axis compression and distortions in deep-cell SMLM.
  • To accurately visualize structures like the ryanodine receptor in cardiac myocytes.

Main Methods:

  • Implementation of a novel 3D SMLM system with drift-free capabilities (<0.7 nm lateral, 2.5 nm axial).
  • Development and application of depth-dependent calibrations to correct for Z-axis distortions.
  • Utilizing a time-domain filter to resolve overlapping single-molecule blinks.

Main Results:

  • Achieved high-accuracy imaging many microns deep into cells.
  • Depth-dependent calibration revealed a significantly greater Z-range for deep receptors compared to conventional methods.
  • Resolved individual 30 nm ryanodine receptors with accuracy comparable to electron tomography.

Conclusions:

  • The novel 3D SMLM system overcomes limitations of deep-cell imaging.
  • Depth-dependent calibration is crucial for accurate Z-localization in SMLM.
  • The system enables detailed structural analysis of cellular components deep within tissues.