Related Experiment Video
Updated: Apr 5, 2026
Production and Visualization of Bacterial Spheroplasts and Protoplasts to Characterize Antimicrobial Peptide Localization
Published on: August 11, 2018
Preparation of bio-deep eutectic solvent triggered cephalopod shaped silver chloride-DNA hybrid material having
Jitkumar Bhatt1, Dibyendu Mondal1, Gopal Bhojani1
1Marine Biotechnology and Ecology Division, CSIR-Central Salt & Marine Chemicals Research Institute, G. B Marg, Bhavnagar 364002, Gujarat, India; AcSIR-Central Salt & Marine Chemicals Research Institute, G. B Marg, Bhavnagar 364002, Gujarat, India.
Abstract:
2.5% w/w DNA (Salmon testes) was solubilized in a bio-deep eutectic solvent [(bio-DES), obtained by the complexation of choline chloride and ethylene glycol at 1:2 molar ratio] containing 1% w/w of silver chloride (AgCl) to yield a AgCl decorated DNA based hybrid material. Concentration dependent formation of AgCl crystals in the DES was observed and upon interaction with DNA it gave formation of a cephalopod shaped hybrid material. DNA was found to maintain its chemical and structural stability in the material. Further, AgCl microstructures were found to have orderly self assembled on the DNA helices indicating the electrostatic interaction between Ag(+) and phosphate side chain of DNA as a driving force for the formation of the material with ordered microstructural distribution of AgCl. Furthermore, the functionalized material exhibited excellent antibacterial and bactericidal activity against both Gram negative and Gram positive pathogenic bacteria.
