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Updated: Apr 5, 2026
Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development
Published on: August 10, 2015
Porcine kidneys as a source of ECM scaffold for kidney regeneration
Yong Guan1, Shuangde Liu2, Yuqiang Liu1
1Department of Urology, The Second Hospital, Shandong University, China.
Objective:
To produce and examine decellularized kidney scaffolds from porcine as a platform for kidney regeneration research.
Methods:
Porcine kidneys were decellularized with sodium dodecyl sulfate solution and Triton X-100 after the blood was rinsed. Then the renal ECM scaffolds were examined for vascular imaging, histology to investigate the vascular patency, degree of decellularization.
Results:
Renal ECM scaffolds of porcine kidneys were successfully produced. Decellularized renal scaffolds retained intact microarchitecture including the renal vasculature and essential extracellular matrix components.
Conclusion:
We have developed an excellent decellularization method that can be used in large organs. These scaffolds maintain their basic components, and show intact vasculature system. This represents a step toward development of a transplantable organ using tissue engineering techniques.
