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Updated: Apr 5, 2026
Symptom Assessment of Patients with Allergic Rhinitis Using an Allergen Exposure Chamber
Published on: March 3, 2023
Expression profile of ADAM10 and ADAM17 in allergic rhinitis
Hee Won Chueh1, Seong Kook Park2, Dae Young Hur3
1Department of Pediatrics, Dong-A University, College of Medicine, Busan, Korea.
Background:
A disintegrin and metalloprotease (ADAM) is a multifunctional gene family that contributes to the homeostasis of the extracellular matrix, transduction of specific intracellular signals, organogenesis, inflammation, tissue remodeling, adhesion, and cell migration. ADAM17 is the best characterized sheddase, with widespread putative substrates, including various inflammatory modulators. ADAM10 is the most similar ADAM to ADAM17 in terms of both protein sequence and the structural properties of their catalytic domains. The objective of this work was to assess the expression of ADAM10 and ADAM17 in allergic rhinitis to gain insight into their respective roles.
Methods:
The expression of ADAM10 and ADAM17 was investigated in the nasal mucosa under allergic and nonallergic conditions. Tissue samples were evaluated by reverse-transcription polymerase chain reaction (RT-PCR) and Western blotting, and data were analyzed semiquantitatively with densitometry.
Results:
The ADAM17 messenger RNA (mRNA) level was significantly (p < 0.001) lower in the allergic nasal mucosa than in the nonallergic nasal mucosa, whereas the ADAM10 mRNA level was significantly (p < 0.001) lower in the nonallergic nasal mucosa. The ADAM17 protein levels were also significantly (p < 0.001) lower in the allergic nasal mucosa, whereas the ADAM10 protein levels were lower in the nonallergic nasal mucosa (p = 0.002).
Conclusion:
Decreased expression of ADAM17 and increased expression of ADAM10 may contribute to the development of allergic rhinitis through unknown pathways. We suggest that understanding the expression profile of ADAM17 and ADAM10 might help to elucidate the mechanism of allergic rhinitis.
Insights
Researchers found decreased ADAM17 and increased ADAM10 expression in allergic rhinitis nasal tissue. This study investigated the roles of ADAM10 and ADAM17 in allergic rhinitis development.
Area of Science:
- Biochemistry
- Immunology
- Molecular Biology
Background:
- A disintegrin and metalloprotease (ADAM) gene family plays roles in extracellular matrix homeostasis, signal transduction, and cell migration.
- ADAM17 is a well-characterized sheddase with numerous substrates involved in inflammation.
- ADAM10 shares structural and sequence similarities with ADAM17, suggesting potential overlapping functions.
Purpose of the Study:
- To investigate the expression levels of ADAM10 and ADAM17 in the nasal mucosa of individuals with allergic rhinitis.
- To elucidate the potential roles of ADAM10 and ADAM17 in the pathogenesis of allergic rhinitis.
Main Methods:
- Expression analysis of ADAM10 and ADAM17 in allergic and non-allergic nasal mucosa samples.
- Utilized reverse-transcription polymerase chain reaction (RT-PCR) for mRNA level assessment.
- Employed Western blotting and densitometry for quantitative protein level analysis.
Main Results:
- ADAM17 mRNA and protein levels were significantly lower in allergic nasal mucosa compared to non-allergic controls.
- ADAM10 mRNA and protein levels were significantly lower in non-allergic nasal mucosa compared to allergic samples.
- Demonstrated differential expression patterns for ADAM10 and ADAM17 in allergic rhinitis.
Conclusions:
- Reduced ADAM17 expression and increased ADAM10 expression may be implicated in allergic rhinitis development via unidentified mechanisms.
- Understanding the distinct expression profiles of ADAM10 and ADAM17 is crucial for deciphering allergic rhinitis mechanisms.
- Further research is warranted to explore the specific pathways influenced by these ADAM proteases in allergic inflammation.
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