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Role of miR-148a in cutaneous squamous cell carcinoma by repression of MAPK pathway

Quan Luo1, Wei Li1, Tian Zhao1

  • 1Department of Dermatology, Guangzhou Institute of Dermatology, Guangzhou, China.

Archives of Biochemistry and Biophysics
|August 9, 2015
PubMed

Insights

MicroRNA-148a (miR-148a) is downregulated in cutaneous squamous cell carcinoma (CSCC). Overexpressing miR-148a inhibits CSCC cell proliferation and metastasis by targeting MAP3K4 and MAP3K9, suggesting its tumor-suppressive role.

Area of Science:

  • Oncology
  • Molecular Biology
  • Gene Regulation

Background:

  • MicroRNA (miRNA) dysregulation is common in human cancers.
  • The role of miR-148a in cutaneous squamous cell carcinoma (CSCC) is largely unknown.
  • miR-148a is frequently downregulated in various cancer types.

Purpose of the Study:

  • To investigate the expression and cellular function of miR-148a in CSCC.
  • To identify target genes of miR-148a in CSCC.
  • To explore the therapeutic potential of miR-148a in CSCC.

Main Methods:

  • Quantitative real-time PCR to assess miR-148a expression in CSCC tissues and cell lines.
  • Bioinformatic prediction and experimental validation of miR-148a target genes (MAP3K2, MAP3K4, MAP3K9).
  • In vitro assays to evaluate the effect of miR-148a overexpression on CSCC cell proliferation and metastasis.

Main Results:

  • miR-148a was significantly underexpressed in CSCC tissues and cell lines.
  • MAP3K4 and MAP3K9 were validated as direct targets of miR-148a in CSCC.
  • Overexpression of miR-148a inhibited CSCC cell proliferation and metastasis by downregulating MAP3K4 and MAP3K9.
  • MAP3K4 and MAP3K9 expression were negatively correlated with miR-148a levels in CSCC tissues.

Conclusions:

  • miR-148a functions as a tumor suppressor in CSCC.
  • The tumor-suppressive activity of miR-148a is mediated through the inhibition of the MAPK pathway via MAP3K4 and MAP3K9.
  • miR-148a represents a potential therapeutic target for CSCC treatment.
Keywords:
CSCCMAP3K4MAP3K9miR-148a

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