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Biosynthesis of Nucleic Acids

Biosynthesis of Nucleic Acids

Nucleic acid biosynthesis is a fundamental biochemical process that produces the purine and pyrimidine nucleotides essential for DNA and RNA synthesis. This pathway maintains a balanced nucleotide pool, preventing imbalances that could jeopardize genetic integrity and cellular function. Given the crucial role of nucleotides, their synthesis is tightly regulated to ensure proper cellular homeostasis.Purine BiosynthesisThe biosynthesis of purine nucleotides begins with ribose-5-phosphate, a...

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Related Experiment Video

Updated: Apr 5, 2026

Free Radicals in Chemical Biology: from Chemical Behavior to Biomarker Development

Free Radicals in Chemical Biology: from Chemical Behavior to Biomarker Development

Published on: April 15, 2013

Pseudouridine Chemical Labeling and Profiling.

Xiaoyu Li¹, Shiqing Ma², Chengqi Yi³

¹State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing, China.

Methods in Enzymology

|August 9, 2015

Summary

This summary is machine-generated.

Pseudouridine (Ψ), an abundant RNA modification, has unknown functions in messenger RNA due to detection challenges. A new method, CeU-Seq, enables comprehensive transcriptome-wide analysis of pseudouridylation for future functional studies.

Keywords:

Chemical pull down Pseudouridine Selective labeling Single-base resolution Transcriptome-wide profiling Whole-genome sequencing mRNA modification

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Syntheses, Crystallization, and Spectroscopic Characterization of 3,5-Lutidine N-Oxide Dehydrate

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Published on: April 24, 2018

Area of Science:

Biochemistry
Molecular Biology
Genomics

Background:

Pseudouridine (Ψ) is the most abundant RNA modification.
Its prevalence and function in messenger RNA (mRNA) remain largely unknown.
Transcriptome-wide detection of Ψ is challenging, hindering research.

Purpose of the Study:

To develop a method for comprehensive transcriptome-wide analysis of pseudouridylation.
To overcome the limitations in detecting pseudouridine in mRNA.
To facilitate future functional studies of Ψ-mediated biological regulation.

Main Methods:

CeU-Seq: A selective chemical labeling and pull-down method.
Enables comprehensive analysis of pseudouridylation across the transcriptome.
Designed to address challenges in pseudouridine detection.

Main Results:

A novel method, CeU-Seq, has been developed for pseudouridine detection.
This method allows for transcriptome-wide analysis of pseudouridylation.
Provides a foundation for future research into Ψ's role.

Conclusions:

CeU-Seq offers a powerful tool for studying pseudouridylation.
The method facilitates the comprehensive analysis of Ψ in mRNA.
Paves the way for understanding Ψ-mediated biological regulation.