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Yes-associated protein (YAP) expression is involved in epithelial-mesenchymal transition in hepatocellular carcinoma

S Wang1,2, H Li1, G Wang1

  • 1Department of Hepatic Surgery, Third Affiliated Hospital of Sun Yat-sen University, No. 600 Tianhe District, Guangzhou, 510630, Guangdong, China.

Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico
|August 9, 2015
PubMed
Abstract

Insights

Downregulating yes-associated protein (YAP) in hepatocellular carcinoma cells reduced invasion and altered epithelial-mesenchymal transition markers. YAP is closely linked to E-cadherin and N-cadherin expression in these cancer cells.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cell Biology

Background:

  • Epithelial-mesenchymal transition (EMT) is a critical process in cancer progression and metastasis.
  • Yes-associated protein (YAP) is a key regulator of cell proliferation and tissue growth, implicated in various cancers.
  • Understanding YAP's role in EMT is crucial for developing targeted cancer therapies.

Purpose of the Study:

  • To investigate the biological impact of downregulating yes-associated protein (YAP) via RNA interference.
  • To examine the effect of YAP downregulation on epithelial-mesenchymal transition (EMT) markers in MHCC97H and MHCC97L hepatocellular carcinoma cell lines.

Main Methods:

  • MHCC97H and MHCC97L cells were transfected with YAP-siRNA.
  • Protein and mRNA levels of E-cadherin and N-cadherin were analyzed using Western blotting and real-time PCR.
  • Cell invasiveness was assessed via Transwell invasion assays.

Main Results:

  • YAP silencing significantly increased E-cadherin expression in MHCC97H and MHCC97L cells.
  • N-cadherin expression decreased in MHCC97H but showed no significant change in MHCC97L cells.
  • Cell invasiveness was significantly reduced in both cell lines after YAP downregulation.

Conclusions:

  • YAP expression is closely associated with EMT markers, specifically E-cadherin and N-cadherin, in hepatocellular carcinoma.
  • Downregulation of YAP impacts EMT and reduces the invasive potential of MHCC97H and MHCC97L cells.
  • The findings suggest YAP as a potential therapeutic target for hepatocellular carcinoma treatment.
Keywords:
Epithelial–mesenchymal transition (EMT)Hepatocellular carcinoma (HCC)YAP (yes-associated protein)

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