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An Improved Method for the Preparation of Type I Collagen From Skin
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Northern pike (Esox lucius) collagen: Extraction, characterization and potential application
J Kozlowska1, A Sionkowska1, J Skopinska-Wisniewska1
1Faculty of Chemistry, Nicolaus Copernicus University in Torun, Gagarina 7, 87-100 Torun, Poland.
International Journal of Biological Macromolecules
|August 9, 2015
Summary
Northern pike scales yield acid soluble collagen (ASC) and pepsin soluble collagen (PSC). These collagens are type I, with high glycine and hydroxyproline content, showing potential for various applications.
Area of Science:
- Biochemistry
- Materials Science
Background:
- Collagen is a vital structural protein with numerous applications.
- Fish scales represent an underutilized source of collagen.
Purpose of the Study:
- To extract and characterize acid soluble collagen (ASC) and pepsin soluble collagen (PSC) from northern pike (Esox lucius) scales.
- To evaluate the potential of northern pike scales as a novel source of collagen.
Main Methods:
- Collagen extraction using acid and pepsin solubilization.
- Characterization using FT-IR and amino acid analysis.
- Analysis of thermal properties (denaturation temperature) and surface properties of collagen films.
- Tensile testing of collagen films.
Main Results:
- Collagen presence confirmed by FT-IR and amino acid analysis, with high glycine and hydroxyproline content.
- Both ASC and PSC were identified as type I collagen.
- ASC films exhibited more polar and less rough surfaces compared to PSC films, with observable fibril formation.
- ASC films demonstrated superior tensile properties over PSC films.
Conclusions:
- Northern pike scales are a viable source for extracting type I collagen.
- The distinct properties of ASC and PSC suggest potential for specialized applications.
- Further research into utilizing northern pike scales for collagen production is warranted.
