Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Reversed-phase chromatographic method development for peptide separations using the computer simulation program

C T Mant1, T W Burke, N E Zhou

  • 1Department of Biochemistry, University of Alberta, Edmonton, Canada.

Journal of Chromatography
|December 27, 1989
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

McGill University.

The Journal of comparative medicine and veterinary archives·2022
Same author

Social support correlates with glucocorticoid concentrations in wild African elephant orphans.

Communications biology·2022
Same author

Nitrate of Silver and Its Application in Diseased Conditions of the Mouth and Teeth.

The American journal of dental science·2019
Same author

Functional consequences of AXL sequence variants in hypogonadotropic hypogonadism.

The Journal of clinical endocrinology and metabolism·2014
Same author

Volatile components in scent gland secretions of garter snakes (Thamnophis spp.).

Journal of chemical ecology·2013
Same author

Modelling of peptide and protein structures.

Amino acids·2013
Same journal

Rapid synthesis of isoprenoid diphosphates and their isolation in one step using either thin layer or flash chromatography.

Journal of chromatography·2013
Same journal

[Electrophoresis of radionuclides on cellogel].

Journal of chromatography·2012
Same journal

Frontal affinity chromatography: theory for its application to studies on specific interactions of biomolecules.

Journal of chromatography·2010
Same journal

Cumulative author, subject, compound and special issue indexes. Volumes 601-650 (1992-1993).

Journal of chromatography·1994
Same journal

Determination of the calcium antagonist SIM6080 in plasma by high-resolution gas chromatography.

Journal of chromatography·1994
Same journal

Processing of envelope polypeptides of herpes simplex virus type 1. Demonstration of variation in different cell lines by high-performance liquid chromatography and radioimmunoprecipitation.

Journal of chromatography·1994
See all related articles

A new computer program, ProDigest-LC, accurately predicts peptide behavior in chromatography, aiding scientists in method development and purification. This tool enhances understanding of peptide separation techniques for researchers and students.

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Computational Biology

Background:

  • Protein digests yield complex peptide mixtures requiring advanced separation techniques.
  • High-performance liquid chromatography (HPLC) is crucial for peptide analysis and purification.
  • Developing optimal HPLC methods for diverse peptides can be challenging and time-consuming.

Purpose of the Study:

  • To introduce ProDigest-LC, a computer program for simulating HPLC separations of peptides.
  • To validate the predictive accuracy of ProDigest-LC for various chromatographic modes and peptide properties.
  • To provide a user-friendly tool for optimizing HPLC method development and as an educational aid.

Main Methods:

  • Development and implementation of simulation algorithms for size-exclusion, cation-exchange, and reversed-phase HPLC.

Related Experiment Videos

  • Utilizing known peptide compositions to predict retention behavior and elution profiles.
  • Testing the program's predictive accuracy using peptide standards with varying sizes, charges, and hydrophobicity.

    • Main Results:

    • ProDigest-LC accurately predicts peptide retention times across different HPLC modes.
    • The program successfully simulates elution profiles, including the effects of gradient rate, flow rate, and sample size.
    • Excellent accuracy was achieved in predicting retention times for peptides with diverse physicochemical properties.

    Conclusions:

    • ProDigest-LC is a valuable, user-friendly tool for scientists and students in peptide chromatography.
    • The program enhances the efficiency of HPLC method development and aids in understanding separation principles.
    • Accurate peptide retention prediction is crucial for the practical application and validation of such computational tools.