Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Brain Structural Abnormalities in Addiction Disorders: Transdiagnostic Meta-Analyses.

Human brain mapping·2026
Same author

Examining Phosphatidylethanol (PEth) Formation Rates Within and Between Subjects in Ex Vivo Whole Blood.

Drug testing and analysis·2026
Same author

Evaluating effects of synchronous music reinforcement on increasing treadmill walking speed in a stepwise fashion.

Journal of the experimental analysis of behavior·2026
Same author

Spatial memory in Alzheimer's disease 5XFAD mice is enhanced by XPO1 inhibitor KPT-330.

GeroScience·2026
Same author

Canagliflozin Reprograms the Aging Hippocampus in Genetically Diverse UM-HET3 Mice and Attenuates Alzheimer's-Like Pathology.

Aging cell·2025
Same author

Canagliflozin reprograms the aging hippocampus in genetically diverse UM-HET3 mice and attenuates Alzheimer's-like pathology.

bioRxiv : the preprint server for biology·2025

Related Experiment Video

Updated: Apr 5, 2026

Chronic Intermittent Ethanol Vapor Exposure Paired with Two-Bottle Choice to Model Alcohol Use Disorder
05:12

Chronic Intermittent Ethanol Vapor Exposure Paired with Two-Bottle Choice to Model Alcohol Use Disorder

Published on: June 23, 2023

1.7K

Ethanol self-administration in mice under a second-order schedule.

Richard J Lamb1, Jonathan W Pinkston2, Brett C Ginsburg3

  • 1Department of Psychiatry, University of Texas Health Science Center at San Antonio, San Antonio, TX 78229, USA; Department of Pharmacology, University of Texas Health Science Center at San Antonio, San Antonio, TX 78229, USA.

Alcohol (Fayetteville, N.Y.)
|August 10, 2015
PubMed
Summary

Long Fixed-Interval (FI) schedules can effectively study ethanol seeking in mice. This method allows researchers to examine medication effects on ethanol-related behaviors without direct drug influence.

Keywords:
AlcoholAlcoholismConditioned reinforcementReinstatementRelapseSelective serotonin reuptake inhibitor

More Related Videos

A Method for Evaluating the Reinforcing Properties of Ethanol in Rats without Water Deprivation, Saccharin Fading or Extended Access Training
07:50

A Method for Evaluating the Reinforcing Properties of Ethanol in Rats without Water Deprivation, Saccharin Fading or Extended Access Training

Published on: January 29, 2017

10.3K
Murine Drinking Models in the Development of Pharmacotherapies for Alcoholism: Drinking in the Dark and Two-bottle Choice
07:31

Murine Drinking Models in the Development of Pharmacotherapies for Alcoholism: Drinking in the Dark and Two-bottle Choice

Published on: January 7, 2019

8.8K

Related Experiment Videos

Last Updated: Apr 5, 2026

Chronic Intermittent Ethanol Vapor Exposure Paired with Two-Bottle Choice to Model Alcohol Use Disorder
05:12

Chronic Intermittent Ethanol Vapor Exposure Paired with Two-Bottle Choice to Model Alcohol Use Disorder

Published on: June 23, 2023

1.7K
A Method for Evaluating the Reinforcing Properties of Ethanol in Rats without Water Deprivation, Saccharin Fading or Extended Access Training
07:50

A Method for Evaluating the Reinforcing Properties of Ethanol in Rats without Water Deprivation, Saccharin Fading or Extended Access Training

Published on: January 29, 2017

10.3K
Murine Drinking Models in the Development of Pharmacotherapies for Alcoholism: Drinking in the Dark and Two-bottle Choice
07:31

Murine Drinking Models in the Development of Pharmacotherapies for Alcoholism: Drinking in the Dark and Two-bottle Choice

Published on: January 7, 2019

8.8K

Area of Science:

  • Behavioral neuroscience
  • Pharmacology
  • Neurobiology

Background:

  • Long Fixed-Interval (FI) schedules, especially second-order schedules, produce significant drug- or ethanol-seeking behavior independent of the substance's direct effects.
  • These schedules are valuable for studying medication impacts on substance-seeking behaviors.
  • While common in primates and rats, their utility in mice, advantageous for genetic and neurobiological research, is less established.

Purpose of the Study:

  • To determine if long FI second-order schedules are effective for studying ethanol-seeking behavior in mice.
  • To assess the temporal pattern of responding under this schedule in mice.
  • To investigate the effects of fluvoxamine on ethanol-seeking behavior maintained by this schedule.

Main Methods:

  • Eight C57BL/6J mice were trained on a second-order schedule involving Fixed Ratio 10 (FR10) responses producing a tone, with FR10s completed under an FI 1800-s schedule for ethanol and tone delivery.
  • Behavioral patterns were analyzed, and the effects of acute and chronic fluvoxamine administration were examined.
  • Extinction procedures were conducted by withholding alcohol and/or tone deliveries.

Main Results:

  • The schedule successfully maintained substantial responding in mice, exhibiting a typical FI temporal pattern with accelerating response rates across the interval.
  • Fluvoxamine administration produced modest effects on responding.
  • Extinction of responding was most rapid when both ethanol and tone deliveries were withheld.

Conclusions:

  • Long FI second-order schedules are a viable and effective method for studying ethanol-seeking behavior in mice.
  • This schedule provides a valuable tool for preclinical research into the neurobiology of addiction and the development of pharmacotherapies.
  • The findings support the use of mice in future research utilizing these schedules to understand ethanol-related behaviors.