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Strain-level visualized analysis of cold-stressed Vibrio parahaemolyticus based on MALDI-TOF mass fingerprinting

Guanhua Xuan1, Juntao Jia2, Ying Chen3

  • 1Food Safety Laboratory, College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.

Microbial Pathogenesis
|August 10, 2015
PubMed

Insights

Cold stress significantly alters Vibrio parahaemolyticus at the strain level, as shown by matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass fingerprinting. This comprehensive study reveals distinct bacterial cold stress responses for improved understanding.

Area of Science:

  • Microbiology
  • Bacterial stress responses
  • Proteomics

Background:

  • Vibrio parahaemolyticus is a significant foodborne pathogen.
  • Bacterial responses to cold stress are crucial for survival and public health.
  • Strain-level variations in stress response are not well understood.

Purpose of the Study:

  • To investigate the impact of cold stress on Vibrio parahaemolyticus at the strain level.
  • To analyze changes in peptide mass fingerprinting profiles under cold conditions.
  • To understand bacterial cold stress responses in a large cohort of strains.

Main Methods:

  • Matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass fingerprinting for peptide profiling.
  • Self-organized map (SOM) classification for data visualization.
  • Cluster analysis to identify distinct strain groupings.

Main Results:

  • Peptide mass fingerprinting profiles of V. parahaemolyticus showed substantial changes under cold stress.
  • SOM classification separated cold-stressed strains into 14 distinct groups, differing from control groups.
  • Significant strain-level variations in response to cold stress were observed.

Conclusions:

  • Cold stress induces significant, strain-specific alterations in Vibrio parahaemolyticus.
  • MALDI-TOF and SOM analysis provide a powerful tool for studying bacterial cold stress.
  • This study enhances the understanding of bacterial cold stress responses in V. parahaemolyticus.
Keywords:
Cold stressMALDI-typingPeptide mass fingerprintingPhyloproteomic analysisSelf-organized mapVibrio parahaemolyticusVisualized analysis

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