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Human Toll like receptor 4 gene expression of PBMCs in diabetes mellitus type 2 patients
Z Sepehri1, Z Kiani2, A A Nasiri1
1Zabol University of Medical Sciences Zabol Iran.
Abstract:
Toll like receptor 4 (TLR4) is one of the most pivotal pathogen recognition receptors (PRRs) in innate immune systems. In this study, we evaluate the expression of the TLR4 in patients with diabetes mellitus type 2 (DM2) in comparison to healthy controls. Expression of TLR4 in 32 human peripheral blood mononuclear cells (PBMCs) of patients with DM2 and 20 control samples was assessed using Real—Time PCR technique. For each patient, body mass index (BMI) and blood glucose levels were measured. The results of Real—Time PCR showed a 5—folds increase in expression of TLR4 on the PBMCs of DM2 patients in comparison to controls. No correlation was observed between the TLR4 expression and sex or BMI. Our results confirmed that DM2 can increase TLR4 expression independent from sex, blood glucose concentrations and BMI.
Insights
Diabetes mellitus type 2 (DM2) significantly elevates Toll-like receptor 4 (TLR4) expression in immune cells. This increase occurs independently of patient sex, blood glucose levels, or body mass index.
Area of Science:
- Immunology
- Endocrinology
- Molecular Biology
Background:
- Toll-like receptor 4 (TLR4) acts as a key pathogen recognition receptor (PRR) in innate immunity.
- Altered immune responses are implicated in the pathogenesis of type 2 diabetes mellitus (DM2).
Purpose of the Study:
- To investigate and compare Toll-like receptor 4 (TLR4) expression levels in patients with type 2 diabetes mellitus (DM2) versus healthy controls.
- To determine potential correlations between TLR4 expression and clinical parameters like BMI and blood glucose.
Main Methods:
- Real-Time PCR was employed to quantify TLR4 expression in peripheral blood mononuclear cells (PBMCs).
- Samples were obtained from 32 DM2 patients and 20 healthy individuals.
- Body mass index (BMI) and blood glucose levels were recorded for each participant.
Main Results:
- A significant five-fold increase in TLR4 expression was observed in PBMCs from DM2 patients compared to controls.
- No statistically significant correlation was found between TLR4 expression levels and sex or BMI.
- Blood glucose concentrations also did not correlate with TLR4 expression levels.
Conclusions:
- Type 2 diabetes mellitus is associated with significantly elevated TLR4 expression in PBMCs.
- The increased TLR4 expression in DM2 is independent of sex, BMI, and blood glucose levels.
- These findings suggest a potential role for TLR4 in DM2 pathophysiology.
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