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Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

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Related Experiment Video

Updated: Apr 5, 2026

In Situ Monitoring of Transiently Formed Molecular Chaperone Assemblies in Bacteria, Yeast, and Human Cells

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Published on: September 2, 2019

Closing in on life: proximity dependent methods for life sciences

Björn Koos¹, Ola Söderberg¹

¹Department of Immunology, Genetics and Pathology, Science for Life Laboratory, Uppsala University, Biomedical Center, Uppsala, Sweden.

|August 11, 2015

Summary

No abstract available in PubMed .

Keywords:

Chromosome Section hybridization chain reaction (HCR)protein-protein interactions proximity ligation assay (PLA)signaling

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