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Updated: Apr 5, 2026
A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
Published on: September 10, 2014
A novel electrochemical biosensor for selective determination of mercury ions based on DNA hybridization
Maryam Ebrahimi1, Jahan Bakhsh Raoof1, Reza Ojani1
1Eletroanalytical Chemistry Research Laboratory, Department of Analytical Chemistry, Faculty of Chemistry, University of Mazandaran, Babolsar, Iran.
Abstract:
An electrochemical biosensor was developed for Hg(2+) determination based on DNA hybridization. In the presence of Hg(2+), the target and probe DNAs with thymine-thymine (T-T) mismatches could hybridize by forming T-Hg(2+)-T complex. This induced DNA hybridization led to the decrease in reduction peak currents of ethyl green (EG) as electroactive label, which could be used for determination of Hg(2+). The difference in the value of the peak currents of EG before and after DNA hybridization (ΔI) was linear with the concentration of Hg(2+) in the range of 9.0 × 10(-11)-1.0 × 10(-9) M. The detection limit was 3.08 × 10(-11) M.
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