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Updated: Apr 5, 2026
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Dissection of Xenopus laevis Neural Crest for in vitro Explant Culture or in vivo Transplantation
Published on: March 4, 2014
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Cdon promotes neural crest migration by regulating N-cadherin localization
Davalyn R Powell1, Jason S Williams1, Laura Hernandez-Lagunas2
1Department of Craniofacial Biology, School of Dental Medicine, Anschutz Medical Campus, University of Colorado, Aurora, CO 80045, USA; Cell Biology, Stem Cells, and Development Graduate Program, Anschutz Medical Campus, University of Colorado, Aurora, CO 80045, USA.
Developmental Biology
|August 11, 2015
Summary
Cdon is crucial for zebrafish neural crest cell migration. Its absence disrupts cell movement by affecting N-cadherin localization, leading to stalled migration and altered cell behavior.
Area of Science:
- Developmental Biology
- Cell Biology
- Neuroscience
Background:
- Neural crest cells (NCCs) are vertebrate-specific embryonic cells vital for development.
- NCC migration is a complex process guided by environmental cues and cell interactions.
Purpose of the Study:
- To investigate the role of cdon, a Sonic hedgehog (Shh) receptor and cell adhesion regulator, in zebrafish neural crest cell migration.
Main Methods:
- Zebrafish model system
- Knockdown of cdon expression
- Live cell imaging
- Transplantation analysis
Main Results:
- cdon is expressed in premigratory NCCs and downregulated upon migration.
- cdon knockdown causes trunk NCCs to stall after exiting the neural tube.
- Loss of cdon leads to reduced migration directionality, increased velocity, and mispositioned cell protrusions.
- cdon is required cell-autonomously for directed NCC migration.
- N-cadherin mislocalization is observed in cdon-knockdown NCCs.
Conclusions:
- cdon plays a novel role in regulating zebrafish neural crest cell migration.
- cdon is essential for proper N-cadherin localization at the cell membrane in migratory NCCs, facilitating directed migration.
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