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Updated: Apr 5, 2026

Biolistic Transformation of a Fluorescent Tagged Gene into the Opportunistic Fungal Pathogen Cryptococcus neoformans
Published on: March 19, 2015
[Interfering effect of an intergenic-derived sRNA of Cryptococcus neoformans]
Objective:
We demonstrated the interfering effect of a new intergenic-derived sRNA of Cryptococcus neoformans.
Methods:
We constructed a library of sRNAs from 20 to 25 nt, and used a reporter gene CLC1 that was fused to the testing sRNAs to show the interference effect, i. e. the degradation of the CLC1 would gene rate an albino phenotype of the transformants.
Results:
Through the CLC-sRNA reporter system, we acquired one sRNA, sRNA-1, located to an intergenic region of the genome of C. neoformans, that displayed interfering effect on CLCl.
Conclusions:
In C. neoformans, the origination of sRNA may be diverse. The endogenous sRNA should play interfering function via the canonical RNAi pathway.
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