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Updated: Oct 8, 2026

Biochemical Reconstitution of Steroid Receptor•Hsp90 Protein Complexes and Reactivation of Ligand Binding
Published on: September 21, 2011
Analysis of glucocorticoid receptor subspecies binding to DNA-cellulose and isolated nuclei
D J Gruol1, K A Wolfe, S Safarian
1Regulatory Biology Laboratory, Salk Institute for Biological Studies, La Jolla, CA 92037.
Abstract:
Conversion of the glucocorticoid receptor into a DNA-binding protein results in the generation of several distinct receptor subspecies (peaks A-E) which can be resolved by anion exchange chromatography. In vitro, the fraction of the receptor population (approx. 40%) which gains a capacity to bind DNA-cellulose is preferentially transformed into the peak A species by a process that was enhanced by the presence of KCl. At 0.4 M KCl, virtually all of the DNA-binding receptor was in the peak A form. Isolated nuclei also exhibit a receptor binding profile similar to that observed with DNA-cellulose.
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