[Overview of methods used for determination of selected sphingolipids in different biological materials]

Sphingolipids are bioactive molecules, which constitute part of the cell membrane. They are involved in signaling and regulation of many cellular processes including cell growth and proliferation, apoptosis, angiogenesis or inflammatory processes. Several methods were described to determine the concentration and conversion of sphingolipids including radioactive labeling, enzymatic methods, MS, RP-HPLC, ESI LC-MS/ MS, UPLC-MS/MS. The development and validation of analytical and extraction methodology for the determination of selected sphingolipids in various biological materials are described in this paper.