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Updated: Apr 5, 2026

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
Published on: October 4, 2019
Towards the commercialization of Botryococcus braunii for triterpenoid production
Khalid A Al-Hothaly1,2, Eric M Adetutu1, Brian H May3
1School of Applied Science, Centre for Environmental Sustainability and Remediation, RMIT University, Bundoora, VIC, 3083, Australia.
Abstract:
Botryococcus braunii can accumulate unusually high levels of triterpenoid hydrocarbons making it a potential source of high value chemicals. However, its commercial application is hampered by its slow growth and lack of large-scale studies of triterpenoid hydrocarbon production. This study investigated hydrocarbon production in two race B of B. braunii strains, Overjuyo-3 and Kossou-4, at 25 °C in 500 L open tanks under artificial lighting in modified BG11 medium over 60 days. Maximum growth was reached by 40 days with Overjuyo-3 producing more biomass (3.05 g L(-1)) than Kossou-4 (2.55 g L(-1)). However, Kossou-4 produced more oil (0.75 g L(-1)) and triterpenoid hydrocarbons (C30-C34; 50 % of oil weight) compared to 0.63 g L(-1) of oil in Overjuyo-3 with triterpenoid hydrocarbons making up 29 % of oil weight. This research demonstrates for the first time that large-scale production of high value triterpenoid hydrocarbon for commercial application is feasible with Kossou-4 strain.
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