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Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
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Updated: Apr 5, 2026

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy

Published on: May 16, 2022

Imaging nano-objects by linear and nonlinear optical absorption microscopies.

Mary Sajini Devadas¹, Tuphan Devkota, Paul Johns

¹Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN 46556-5670, USA.

|August 13, 2015

Summary

This summary is machine-generated.

Absorption based microscopy techniques offer unique insights into nanomaterials. This review covers transient absorption microscopy, photothermal heterodyne imaging, and spatial modulation spectroscopy for advanced imaging and detection applications.

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Area of Science:

* Nanotechnology and Advanced Materials Science
* Optical Spectroscopy and Microscopy

Background:

* Absorption based microscopy is a rapidly developing field for nanomaterial characterization.
* Conventional emission or scattering microscopy techniques have limitations in certain applications.

Purpose of the Study:

* To review and compare three key absorption based microscopy techniques.
* To highlight their applications in nanomaterial imaging and detection.
* To discuss their unique advantages over conventional methods.

Main Methods:

* Discussion of transient absorption microscopy.
* Explanation of photothermal heterodyne imaging.
* Overview of spatial modulation spectroscopy.

Main Results:

* Detailed comparison of the three absorption based techniques.
* Examples of their application in nanomaterial studies from the authors' lab.
* Analysis of the unique information obtainable via these methods.

Conclusions:

* Absorption based microscopy provides complementary information to emission/scattering techniques.
* These methods are powerful tools for imaging and detecting nanomaterials.
* Each technique offers distinct advantages for specific research questions.