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Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR01:59

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR01:59

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CRISPR and crRNAs02:53

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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Homologous Recombination02:31

Homologous Recombination

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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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Related Experiment Video

Updated: Apr 5, 2026

Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes
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Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes

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CRISPR EATING on a Low Budget.

Hatice S Kaya-Okur1, Andrew S Belmont1

  • 1Department of Cell and Developmental Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.

Developmental Cell
|August 13, 2015
PubMed
Summary

Researchers developed CRISPR EATING, a simple and affordable method for creating complex guide RNA libraries. This technique supports various CRISPR/Cas9 applications, including genetic screens and genome manipulation.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • CRISPR/Cas9 technology enables precise genome editing.
  • Generating complex guide RNA libraries is crucial for advanced CRISPR applications.
  • Existing methods for library generation can be complex and costly.

Purpose of the Study:

  • To introduce a simplified and inexpensive method for generating guide RNA libraries.
  • To facilitate complex CRISPR/Cas9 applications such as genetic screens and genome manipulation.
  • To provide a broadly accessible tool for researchers in molecular biology and genetics.

Main Methods:

  • The study describes a novel technique termed "CRISPR EATING".
  • This method focuses on the simplified and cost-effective generation of guide RNA libraries.

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  • The generated libraries are suitable for a wide range of CRISPR/Cas9-based applications.

    • Main Results:

    • Successful generation of complex guide RNA libraries using the CRISPR EATING technique.
    • Demonstration of the technique's applicability to genome visualization and manipulation.
    • Validation of the method for use in large-scale genetic screens.

    Conclusions:

    • CRISPR EATING offers a simplified and economical approach to guide RNA library construction.
    • The technique enhances the accessibility of advanced CRISPR/Cas9 tools for the research community.
    • This method is poised to accelerate discoveries in functional genomics and genome engineering.