Application of Gelatin-Coated Magnetic Particles for Isolation of Genomic DNA from Bones
Related Experiment Video
Updated: Apr 5, 2026
Optimized Bone Sampling Protocols for the Retrieval of Ancient DNA from Archaeological Remains
Published on: November 30, 2021
Objective:
To develop a method for human genomic DNA extraction from bone using gelatin-coated magnetic particles.
Material And Method:
Thirty human metacarpal with the bone age ranging from 36 to 93 years were included in the present study. Genomic DNA was extracted from bones using gelatin-coated magnetic particles. The concentration and purity of DNA were analyzed in comparison with a reference method. In addition, the quality of extracted DNA was examined for sex determination by conventional polymerase chain reaction (PCR).
Results:
The average DNA concentration using gelatin coated magnetic particles exhibited approximately 15 times higher than a reference method with an insignificantly difference of the DNA purity in both methods. Twelve (40%) and fifteen (50%) samples out of thirty DNA isolated using established and reference method, respectively, could be amplified and sex correctly determined by PCR.
Conclusion:
Gelatin coated magnetic particle is rapid, simple, and well-suited for isolation of DNA from bones.
More Related Videos
Related Concept Videos
01:24DNA Isolation
01:34DNA Isolation
02:35DNA Agarose Gel Electrophoresis
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...