[The effect of MTB antigens on the NFAT5 expression in monocytes from human peripheral blood]
Yanhua Liu1, Ruo Wang1, Xiaoxing Cheng2
1Army Tuberculosis Prevention and Control Key Laboratory, Beijing Key Laboratory of New Techniques of Tuberculosis Diagnosis and Treatment, Institute for Tuberculosis Research, the 309th Hospital of Chinese PLA, Beijing 100091, China.
Objective:
To study the effect of Mycobacterium tuberculosis antigens early secretory antigen target 6/culture filtrate protein 10 (ESAT-6/CFP-10) on nuclear factor of activated T-cells 5 (NFAT5) mRNA in monocytes from human peripheral blood.
Methods:
Fifteen patients with active pulmonary tuberculosis (age range: 33-42 y, mean: 37 y, male/female: 9/6) were recruited from the Institute of Tuberculosis in 309 Hospital, and 15 healthy subjects (age range: 37-42 y, mean: 40 y, male/female: 9/6) were recruited from Medical Examination Center of the hospital. Monocytes were isolated from peripheral blood and stimulated with whole cell lysate of H37Rv strain and peptide pool of ESAT-6/CFP-10. Fluorescence quantitative PCR was used to detect the mRNA expression of NFAT5, and differences of NFAT5 mRNA between unstimulated and stimulated monocytes were analyzed by paired t test statistic method.
Results:
The expression of NFAT5 mRNA in monocytes from patients with active pulmonary tuberculosis was significantly decreased when stimulated with peptide pool of ESAT-6/CFP-10 compared with unstimulated monocytes (0.18 ± 0.12 vs. 0.23 ± 0.15) (t = 2.591, P < 0.05). Similarly, the expression of NFAT5 mRNA in monocytes from healthy subjects was also significantly decreased when stimulated with peptide pool of ESAT-6/CFP-10 compared with unstimulated monocytes (0.17 ± 0.09 vs 0.21 ± 0.09) (t = 2.828, P < 0.05). However, the expression of NFAT5 mRNA in monocytes stimulated with whole cell lysate was not significantly changed (t = 1.142, P > 0.05), which was 0.28 ± 0.25 and 0.23 ± 0.15 in stimulated and unstimulated monocytes, respectively. Similarly, the expression of NFAT5 mRNA in monocytes from healthy subjects was also not significantly changed when stimulated with whole cell lysate (t = 0.451 9, P > 0.05), which was 0.20 ± 0.12 and 0.21 ± 0.09 in stimulated and unstimulated monocytes, respectively.
Conclusions:
The MTB antigen ESAT-6/CFP-10 can inhibit NFAT5 expression in human monocytes in vitro.
More Related Videos
10:43Polarization of M1 and M2 Human Monocyte-Derived Cells and Analysis with Flow Cytometry upon Mycobacterium tuberculosis Infection
Published on: September 18, 2020
16:07Determination of the Relative Potency of an Anti-TNF Monoclonal Antibody mAb by Neutralizing TNF Using an In Vitro Bioanalytical Method
Published on: September 16, 2017
