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Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
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RNA-seq03:21

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
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Sanger Sequencing01:57

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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
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Maxam-Gilbert Sequencing01:05

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In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
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Related Experiment Video

Updated: Apr 5, 2026

Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing RIPiT-Seq
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Improved Protocols for Illumina Sequencing.

Iraad F Bronner1, Michael A Quail1, Daniel J Turner2

  • 1Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire, United Kingdom.

Current Protocols in Human Genetics
|August 14, 2015
PubMed
Summary
This summary is machine-generated.

This study details protocol enhancements for Illumina sequencing, improving reliability and data yield in high-throughput settings. Key improvements include reduced amplification bias and optimized insert size distribution for more consistent results.

Keywords:
Illuminanext-generationprotocolssequencer

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Area of Science:

  • Genomics
  • Molecular Biology
  • Biotechnology

Background:

  • Standard Illumina sequencing protocols face challenges in high-throughput environments.
  • Amplification bias and variable insert sizes can impact data quality and yield.

Purpose of the Study:

  • To present protocol modifications for Illumina sequencing.
  • To enhance reliability, reduce bias, and optimize insert size distribution.
  • To ensure consistent high data yields in high-throughput sequencing.

Main Methods:

  • Implementation of optimized library preparation steps.
  • Refinement of amplification and size selection protocols.
  • Validation of modified protocols in a high-throughput setting.

Main Results:

  • Demonstrated increased reliability of the sequencing process.
  • Quantified reduction in amplification bias.
  • Achieved a narrower distribution of insert sizes.
  • Confirmed consistent high yields of sequencing data.

Conclusions:

  • The described protocol improvements enhance the performance of Illumina sequencing.
  • These modifications are crucial for robust high-throughput genomic applications.
  • Optimized protocols lead to more dependable and higher quality sequencing data.