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Creation and Transplantation of an Adipose-derived Stem Cell ASC Sheet in a Diabetic Wound-healing Model
Published on: August 4, 2017
Combination Growth Factor Therapy via Electrostatically Assembled Wound Dressings Improves Diabetic Ulcer Healing In
Benjamin D Almquist1,2,3,4, Steven A Castleberry2,3,4, Julia B Sun2,3,4
1Department of Bioengineering, Imperial College London, London, SW7 2AZ, UK.
Abstract:
Chronic skin ulcerations are a common complication of diabetes mellitus, affecting up to one in four diabetic individuals. Despite the prevalence of these wounds, current pharmacologic options for treating them remain limited. Growth factor-based therapies have displayed a mixed ability to drive successful healing, which may be due to nonoptimal delivery strategies. Here, a method for coating commercially available nylon dressings using the layer-by-layer process is described to enable both sustained release and independent control over the release kinetics of vascular endothelial growth factor 165 and platelet-derived growth factor BB. It is shown that the use of strategically spaced diffusion barriers formed spontaneously by disulfide bonds enables independent control over the release rates of incorporated growth factors, and that in vivo these dressings improve several aspects of wound healing in db/db mice.
Insights
Diabetic wound healing is improved by novel nylon dressings that control the release of vascular endothelial growth factor and platelet-derived growth factor. This innovative delivery system enhances chronic ulcer treatment for diabetic individuals.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Wound Healing
Background:
- Chronic skin ulcerations are a significant complication of diabetes mellitus, impacting a substantial portion of patients.
- Current treatments for diabetic wounds have limitations, and growth factor therapies show variable success due to delivery challenges.
Purpose of the Study:
- To develop a novel drug delivery system for sustained and controlled release of growth factors for diabetic wound healing.
- To investigate the efficacy of layer-by-layer coated nylon dressings for enhanced wound repair.
Main Methods:
- Utilized a layer-by-layer coating process on nylon dressings to incorporate vascular endothelial growth factor 165 (VEGF165) and platelet-derived growth factor BB (PDGF-BB).
- Engineered diffusion barriers using disulfide bonds to achieve independent control over growth factor release kinetics.
- Evaluated the in vivo efficacy of the developed dressings in a db/db mouse model of diabetic wound healing.
Main Results:
- Demonstrated sustained release and independent control over the release rates of VEGF165 and PDGF-BB from the coated dressings.
- The developed dressings significantly improved multiple aspects of wound healing in diabetic mice (db/db model).
Conclusions:
- The developed layer-by-layer coated nylon dressings offer a promising strategy for controlled delivery of multiple growth factors.
- This innovative approach holds potential for improving the treatment of chronic diabetic skin ulcerations.
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