Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Assembly of Cytoskeletal Filaments01:18

Assembly of Cytoskeletal Filaments

28.4K
Cytoskeletal filaments are polymeric forms of smaller protein subunits. However, individual cytoskeletal filaments may easily disassemble or associate with other similar filaments to form rigid structures. Microfilaments, made of actin monomers, rely on actin-binding proteins to form bundles and create networks of individual actin filaments. Microtubules rely on microtubule-associated proteins (MAPs) to form sturdy cylindrical structures. However, the proteins involved in forming complex...
28.4K
Cytoskeletal Accessory Proteins01:13

Cytoskeletal Accessory Proteins

4.3K
The cytoskeleton is an essential cell component that plays several structural and functional roles. However, the filaments that make up the cytoskeleton cannot function independently and depend on the accessory or ancillary proteins to effectively carry out their function. Accessory proteins associate with cytoskeletal filaments and their monomers, aiding filament formation and function. They also help in the cross-communication among cytoskeletal filaments. Cytoskeletal accessory proteins are...
4.3K
Formation of Intermediate Filaments00:57

Formation of Intermediate Filaments

4.2K
Intermediate filaments are cytoskeletal proteins with higher tensile strength and flexibility than microfilaments and microtubules. Unlike the other two cytoskeletal proteins, intermediate filament formation lacks the enzymatic activity to hydrolyze nucleotides like ATP and GTP to generate energy for polymerization. Therefore, the formation of intermediate filaments is multistep self-assembly. The involvement of any accessory proteins in intermediate filament formation has not yet been...
4.2K
The Structure of Intermediate Filaments01:19

The Structure of Intermediate Filaments

6.0K
The intermediate filaments are one of three widely studied cytoskeletal filaments. They are so named as their diameter (10 nm) is in between that of microfilaments (7 nm) and the microtubules (25 nm).  These filaments are highly stable and can remain intact when exposed to high salt concentrations and detergents. These filaments are responsible for providing stability and mechanical support to the cells. They also help in cell adhesion and maintaining tissue integrity.
Intermediate...
6.0K
Adaptability of Cytoskeletal Filaments01:12

Adaptability of Cytoskeletal Filaments

6.9K
The cytoskeleton is a complex dynamic structure performing varied functions based on cellular requirements. The adaptability of the individual filaments in the cytoskeleton determines their ability to perform various functions within the cell. It can undergo rapid reorganization during processes like cell division or remain stable for several hours as in the interphase. The adaptability of these filaments depends on stringent regulatory mechanisms. The microfilament and microtubules of the...
6.9K
Formation of Higher-order Actin Filaments01:11

Formation of Higher-order Actin Filaments

3.8K
The polymerization of G-actin monomers into filamentous F-actin is a multi-step process. Once the F-actins are formed, they can bundle together in different arrangements to form higher-order networks and regulate cellular functions. Common examples include the formation of lamellipodia and filopodia at the cell's leading edge by actin reorganization in a migrating cell. The microvilli on the brush border epithelial cells are also formed through the F-actin network.
The high-order actin...
3.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

L-type pyocins inhibit the BAM complex to kill without cell entry.

Nature communications·2026
Same author

A Novel Pilus System in Candidate Phyla Radiation Bacteria.

bioRxiv : the preprint server for biology·2026
Same author

Aerobic soil bacteria adapt to hypoxia by hybridizing fermentation with carbon storage.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

An Asgard archaeon from a modern analog of ancient microbial mats.

Current biology : CB·2026
Same author

Antiparallel stacking of Csu pili drives Acinetobacter baumannii 3D biofilm assembly.

Nature communications·2026
Same author

Molecular structure of the ESCRT-III-based archaeal CdvAB cell division machinery.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Scalable phosphotyrosine enrichment with SH2 superbinder enables deep profiling of EGF responses.

The EMBO journal·2026
Same journal

Essential nucleus-apical pole linkage maintains division fidelity during Plasmodium progeny formation.

The EMBO journal·2026
Same journal

From cell atlases to mechanisms: bridging scRNA-seq discovery with in vivo genetics.

The EMBO journal·2026
Same journal

Mitochondrial calcium regulates lipid metabolism by modulating tethering of mitochondria to lipid droplets.

The EMBO journal·2026
Same journal

Chromosome condensation mechanically primes the nucleus for mitosis.

The EMBO journal·2026
Same journal

NDR kinase SAX-1 controls dendrite branch-specific elimination during neuronal remodeling in C. elegans.

The EMBO journal·2026
See all related articles

Related Experiment Video

Updated: Apr 5, 2026

Using Microfluidics and Fluorescence Microscopy to Study the Assembly Dynamics of Single Actin Filaments and Bundles
08:02

Using Microfluidics and Fluorescence Microscopy to Study the Assembly Dynamics of Single Actin Filaments and Bundles

Published on: May 5, 2022

3.2K

Collaborative protein filaments.

Debnath Ghosal1, Jan Löwe2

  • 1MRC Laboratory of Molecular Biology, Cambridge, UK dghosal@caltech.edu.

The EMBO Journal
|August 14, 2015
PubMed
Summary
This summary is machine-generated.

Prokaryotic cells utilize collaborative filaments, proteins that polymerize with matrices like DNA, for essential functions. This review categorizes these matrix-assisted systems and explores their roles in cellular processes.

Keywords:
DNA/membrane‐assisted filamentsactin/tubulin cytoskeletonbacterial cytoskeletoncytomotivematrix‐assisted filaments

More Related Videos

Probing Myosin Ensemble Mechanics in Actin Filament Bundles Using Optical Tweezers
06:53

Probing Myosin Ensemble Mechanics in Actin Filament Bundles Using Optical Tweezers

Published on: May 4, 2022

2.7K
Visualizing Actin and Microtubule Coupling Dynamics In Vitro by Total Internal Reflection Fluorescence TIRF Microscopy
08:44

Visualizing Actin and Microtubule Coupling Dynamics In Vitro by Total Internal Reflection Fluorescence TIRF Microscopy

Published on: July 20, 2022

4.1K

Related Experiment Videos

Last Updated: Apr 5, 2026

Using Microfluidics and Fluorescence Microscopy to Study the Assembly Dynamics of Single Actin Filaments and Bundles
08:02

Using Microfluidics and Fluorescence Microscopy to Study the Assembly Dynamics of Single Actin Filaments and Bundles

Published on: May 5, 2022

3.2K
Probing Myosin Ensemble Mechanics in Actin Filament Bundles Using Optical Tweezers
06:53

Probing Myosin Ensemble Mechanics in Actin Filament Bundles Using Optical Tweezers

Published on: May 4, 2022

2.7K
Visualizing Actin and Microtubule Coupling Dynamics In Vitro by Total Internal Reflection Fluorescence TIRF Microscopy
08:44

Visualizing Actin and Microtubule Coupling Dynamics In Vitro by Total Internal Reflection Fluorescence TIRF Microscopy

Published on: July 20, 2022

4.1K

Area of Science:

  • Cell Biology
  • Microbiology
  • Biochemistry

Background:

  • Prokaryotic cells possess dynamic cytoskeletal filaments crucial for cellular functions.
  • While many filaments self-assemble, some proteins require a matrix (DNA, lipid membrane, or another filament) for polymerization.
  • These matrix-assisted filament systems are often nucleotide-dependent and cytomotive but are seldom recognized as part of the bacterial cytoskeleton.

Purpose of the Study:

  • To categorize matrix-assisted filament-forming systems in prokaryotes.
  • To introduce a simple nomenclature for these systems, termed "collaborative filaments".
  • To highlight common principles and functions of collaborative filaments in both prokaryotes and eukaryotes.

Main Methods:

  • Literature review and synthesis of existing research on prokaryotic filament systems.
  • Categorization of filament systems based on their dependency on a matrix for polymerization.
  • Comparative analysis of matrix-assisted filaments in prokaryotes and eukaryotes.

Main Results:

  • Identification and categorization of a class of filament systems named "collaborative filaments".
  • Demonstration that collaborative filaments are prevalent in both prokaryotic and eukaryotic organisms.
  • Association of collaborative filaments with critical cellular processes such as chromosome segregation, DNA repair, gene silencing, and cytokinesis.

Conclusions:

  • Collaborative filaments represent a significant, yet underappreciated, component of cellular machinery.
  • Understanding the principles governing collaborative filaments is key to comprehending vital cellular functions.
  • This framework provides a basis for future research into these essential biological systems.